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The effect of ascorbic acid on infection of chick-embryo ciliated tracheal organ cultures by coronavirus


Chick embryo tracheal organ cultures showed increased resistance to infection by a coronavirus after exposure to ascorbate, while chick respiratory epithelium and allantois-on-shell preparations showed no increase in resistance to infection by an influenza virus or a paramyxovirus.


  1. 1.

    Anderson, T. W., Beaton, G. G., Corey, P., Spero, L.: Winter illness and vitamin C: The effect of relatively low dose. Canad. Med. Assoc. J.112, 823–826 (1975).

    Google Scholar 

  2. 2.

    Bolin, D. W., Book, L.: Oxidation of ascorbic acid to dehydroascorbic acid. Science106, 451 (1947).

    Google Scholar 

  3. 3.

    Chalmers, T. C.: Effects of ascorbic acid on the common cold: An evaluation of the evidence. Amer. J. Med.58, 532–536 (1975).

    Google Scholar 

  4. 4.

    Cherry, J. D., Taylor-Robinson, D.: Large-quantity production of chicken embryo tracheal organ cultures and use in viral and mycoplasma studies. Appl. Microbiol.19, 658–662 (1970).

    Google Scholar 

  5. 5.

    Dykes, M. H., Meier, P.: Ascorbic acid and the common cold: Evaluation of its efficacy and toxicity. J. Amer. Med. Assoc.231, 1073–1079 (1975).

    Google Scholar 

  6. 6.

    Fazekas, de St.Groth, S., White, D. O.: An improved assay for the infectivity of influenza viruses. J. Hyg.56, 151–163 (1958).

    Google Scholar 

  7. 7.

    Fenner, F.: Classification and nomenclature of viruses. Second Report of the International Committee on Taxonomy of Viruses. Intervirology7, 4–115 (1976).

    Google Scholar 

  8. 8.

    Geber, W. F., Lefkowitz, S. S., Hung, C. Y.: Effect of ascorbic acid, sodium salicylate and caffeine on the serum interferon level in response to viral infection. Pharmacology13, 228–233 (1975).

    Google Scholar 

  9. 9.

    Karlowski, T. R., Chalmers, T. C., Frenkel, L. D., Kapikian, A. Z., Lewis, T. L., Lynch, J. M.: Ascorbic acid for the common cold: A prophylactic and therapeutic trial. J. Amer. Med. Assoc.231, 1038–1042 (1975).

    Google Scholar 

  10. 10.

    Moehring, J. M., Stinebring, W. R., Merchant, D. J.: Survey of interferon production and sensitivity in human cell lines. Appl. Microbiol.22, 102–105 (1971).

    Google Scholar 

  11. 11.

    Murphy, B. L., Krushak, D. H., Maynard, J. E., Bradley, D. W.: Ascorbic acid (vitamin C) and its effects on parainfluenza type 3 virus infection in cotton-topped marmosets. Lab. Anim. Sci.24, 229–232 (1974).

    Google Scholar 

  12. 12.

    Reed, L. J., Muench, H.: A simple method of estimating fifty per cent end-points. Amer. J. Hygiene27, 493–497 (1938).

    Google Scholar 

  13. 13.

    Schwartz, A. R., Togo, Y., Hornick, R. B., Tominaga, S., Gleckman, R. A.: Evaluation of the efficacy of ascorbic acid in prophylaxis of induced rhinovirus 44 infection in man. J. inf. Dis.128, 500–505 (1973).

    Google Scholar 

  14. 14.

    Schwerdt, P. R., Schwerdt, C. E.: Effect of ascorbic acid on rhinovirus replication in W 1–38 cells. Prof. Soc. exp. Biol. Med.148, 1237–1243 (1975).

    Google Scholar 

  15. 15.

    Siegel, B. V.: Enhanced interferon response to murine leukemia virus by ascorbic acid. Infect. Immun.10, 409–410 (1974).

    Google Scholar 

  16. 16.

    Willis, R. J., Kratzing, C. C.: Ascorbic acid in rat lung. Biochem. biophys. Res. Comm.59, 1250–1253 (1974).

    Google Scholar 

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Atherton, J.G., Kratzing, C.C. & Fisher, A. The effect of ascorbic acid on infection of chick-embryo ciliated tracheal organ cultures by coronavirus. Archives of Virology 56, 195–199 (1978).

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  • Infectious Disease
  • Influenza
  • Ascorbic Acid
  • Influenza Virus
  • Organ Culture