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Rubella virus identification in primary and continuous monkey kidney cell cultures by immunoperoxidase technique

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Summary

Immunoperoxidase technique was applied to identification of rubella virus isolated from clinical specimens in monkey kidney cell cultures. Findings were confirmed by neutralization tests and by the indirect fluorescent antibody technique.

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References

  1. Avrameas, S.: Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry6, 43–52 (1969).

    Google Scholar 

  2. Benjamin, D. H.: Rapid typing of herpes simplex virus strains using the indirect immunoperoxidase method. Appl. Microbiol.28, 568–571 (1974).

    Google Scholar 

  3. Benjamin, D. R., Ray, C. G.: Use of immunoperoxidase for the rapid identification of human myxoviruses and paramyxoviruses in tissue culture. Appl. Microbiol.28, 47–51 (1974).

    Google Scholar 

  4. Dubois-Dalcq, M., Barbosa, L. H.: Immunoperoxidase stain of measles antigen in tissue culture. J. Virol.12, 909–918 (1973).

    Google Scholar 

  5. Gerna, G., Vasquez, A., McCloud, D. C., Chambers, R. W.: Immunoperoxidase technique for rapid human cytomegalovirus identification. Arch. Virol. (submitted).

  6. Graham, R., Karnovsky, M.: The early stages of absorption of injected horseradish peroxidase in the proximal tubule of mouse kidney: ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem.14, 291–306 (1966).

    Google Scholar 

  7. Hebert, G. A., Pelham, P. L., Pittman, B.: Determination of the optimal ammonium sulfate concentration for the fractionation of rabbit, sheep, horse and goat antisera. Appl. Microbiol.25, 26–36 (1973).

    Google Scholar 

  8. Holmes, I. H., Wark, M. C., Jack, I., Grutzner, J.: Identification of two possible types of virus particle in rubella infected cells. J. gen. Virol.2, 37–42 (1968).

    Google Scholar 

  9. Murphy, F. A., Halonen, P. E., Harrison, A. K.: Electron microscopy of development of rubella virus in BHK21 cells. J. Virol.2, 1223–1227 (1968).

    Google Scholar 

  10. Parkman, P. D., Hopps, H. E., Meyer, H. M., Jr.: Virus isolation procedures. In:Friedman, H., Prier, J. E. (eds.), Rubella pp. 33–50. C. C. Thomas: Springfield, III., 1973.

    Google Scholar 

  11. Schmidt, N. J., Lennette, E. H., Woodie, J. D., Ho, H. H.: Identification of rubella virus isolates by immunofluorescent staining and a comparison of sensitivity of three cell cultures systems for recovery of virus. J. lab. clin. Med.68, 502–509 (1966).

    Google Scholar 

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Gerna, G. Rubella virus identification in primary and continuous monkey kidney cell cultures by immunoperoxidase technique. Archives of Virology 49, 291–295 (1975). https://doi.org/10.1007/BF01317547

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  • DOI: https://doi.org/10.1007/BF01317547

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