Summary
Avian infectious bronchitis virus grownin ovo was purified by differential centrifugation and isopycnic sedimentation in density gradients. The purified virus was analysed by SDS polyacrylamide gel electrophoresis and found to comprise up to sixteen polypeptides, four of which were glycopeptides. Bromelain treatment of the particles removed three polypeptides and two glycopeptides.
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Berry, D. M., Almeida, J. D.: The morphological and biological effects of various antisera on avian infectious bronchitis virus. J. gen. Virol.3, 97–102 (1968).
Burton, K.: A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J.62, 315–323 (1956).
Compans, R. W., Klenk, H. D., Caliguiri, L. A., Choppin, P.W.: Influenza virus proteins. I. Analysis of polypeptides of the virion and identification of spike glycoproteins. Virology42, 880–889 (1970).
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., Smith, F.: Colorimetric method for determination of sugars and related substances. Anal. Chem.28, 350–356 (1956).
Hierholzer, J. C., Palmer, E. L., Whitfield, S. G., Kaye, H. S., Dowdle, W. R.: Protein composition of coronavirus OC43. Virology48, 516–527 (1972).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951).
McIntosh, K.: Coronaviruses: a comparative review. Current Topics in Microbiology and Immunology63, 85–129 (1974).
Mejbaum, W.: The determination of small amounts of pentose, especially in adrenal acid derivates. Hoppe-Seyler's Z. physiol. Chem.258, 117–120 (1939).
Moore, N. F., Kelly, D. C.: Sendai virus structural proteins: analysis of polypeptides linked with disulphide bonds. Intervirology2, 128–133 (1973/74).
Tannock, G. A.: The nucleic acid of infectious bronchitis virus. Arch. ges. Virusforsch.43, 259–271 (1973).
Taylor-Robinson, D.: Personal communication (1973).
Tyrrell, D. A. J., Almeida, J. D., Berry, D. M., Cunningham, C. H., Hamre, D., Hofstad, M. S., Malluci, L., McIntosh, K.: Coronaviruses. Nature, Lond.220, 650 (1968).
Warburg, O., Christian, W.: Isolierung und Kristallisation des Gärungsferments Enolase. Biochem. Z.310, 384–421 (1941).
Watkins, H., Reeve, P., Alexander, D. J.: The ribonucleic acid of infectious bronchitis virus. Archives of Virology47, 279–288 (1975).
Weber, K., Osborn, M.: The reliability of molecular weight determinations by dodecyl sulphate—polyacrylamide gel electrophoresis. J. biol. Chem.244, 4406–4412 (1969).
Zacharius, R. M., Zell, T. E., Morrison, J. H., Woodlock, J. J.: Glycoprotein staining following electrophoresis on acrylamide gels. Anal. Biochem.30, 148–152 (1969).
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Bingham, R.W. The polypeptide composition of avian infectious bronchitis virus. Archives of Virology 49, 207–216 (1975). https://doi.org/10.1007/BF01317539
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DOI: https://doi.org/10.1007/BF01317539