Summary
In the hemagglutination-inhibition test, simple relationships exist between units of hemagglutinating virus and hemagglutination-inhibition antibody titers. Equations have worked out which allow estimating antibody titers for different virus doses, provided all other parameters are kept constant. They are applicable when the isofixation lines in log-log plots as obtained in box titrations either are positioned at angles of 45° or deviate from this value. Using lower or higher doses of hemagglutinin in the test, the coefficient of variation of the estimated antibody titers was found to be approximately 4 per cent. This procedure is useful in a number of virus-cell-serum systems and allows comparisons of antibody titers obtained in different laboratories.
Similar content being viewed by others
References
Feldman, H. A.: Removal by heparin-MnCl2 of nonspecific rubella hemagglutinin serum inhibitor. Proc. Soc. exp. Biol. Med.127, 570–573 (1968).
Hirst, G. K.: The agglutination of red cells by allantoic fluid of chick embryos infected with influenza virus. Science94, 22–23 (1941).
Hirst, G. K.: The quantitative determination of influenza virus and antibodies by means of red cell agglutination. J. exp. Med.75, 49–64 (1942).
Horváth, S., Szöllösy, E., Ivánovics, G.: Distribution of the receptor substance of the influenza and related viruses in the tissue-elements of different animal species. I. The virus adsorbing capacity and agglutinability of the erythrocytes of various vertebrates. Acta Phys. Hung.2, 77–86 (1952).
Liebhaber, H.: Measurement of rubella antibody by hemagglutination inhibition. I. Variables affecting rubella hemagglutination. J. Immunol.104, 818–825 (1970).
McClelland, L., Hare, R.: Adsorption of influenza virus by red cells and newin vitro method of measuring antibodies for influenza virus. Canad. Publ. Health J.32, 530–538 (1941).
Norrby, E.: Hemagglutination by measles virus. 4. A simple procedure for production of high potency antigen for hemagglutination-inhibition (HI) tests. Proc. Soc. exp. Biol. Med.11, 814–818 (1962).
Sever, J. L.: Application of a microtechnique to viral serological investigations. J. Immunol.88, 320–329 (1962).
Stewart, G. L., Parkman, P. D., Hopps, H. E., Douglas, R. D., Hamilton, J. P., Meyer, H. M., Jr.: Rubella-virus hemagglutination-inhibition test. New Engl. J. Med.276, 554–557 (1967).
Author information
Authors and Affiliations
Additional information
With 3 Figures
Rights and permissions
About this article
Cite this article
Horváth, S. Estimation of hemagglutination-inhibition titers. Archives of Virology 49, 199–205 (1975). https://doi.org/10.1007/BF01317538
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01317538