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Isoelectric focusing of herpes simplex virus

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Summary

Herpes simplex virus was inserted into a preformed stable pH gradient and electrofocused for about one hour. Dextran was used as the density gradient forming agent. Virions banded at pH 4.9±0.1 and nucleocapsids at 4.1±0.05. About 10–20 per cent of infective virus was recovered.

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References

  1. Gibson, W., Roizman, B.: Compartmentalization of spermine and spermidine in the herpes simplex virion. Proc. nat. Acad. Sci. U.S.68, 2818–2821 (1971).

    Google Scholar 

  2. Haase, A. T., Baringer, J. R.: The structural polypeptides of RNA slow viruses. Virology57, 238–250 (1974).

    Google Scholar 

  3. Hung, P. P., Robinson, H. L., Robinson, W. S.: Isolation and characterization of proteins from Rous sarcoma virus. Virology43, 251–266 (1971).

    Google Scholar 

  4. Kobayashi, Y., Maudsley, D. V.: Practical aspects of liquid scintillation counting. In: Methods of biochemical analysis (Glick, D., ed.)17, 55–133. New York: John Wiley and Sons, 1969.

    Google Scholar 

  5. Landel, A. M., Aloni, Y., Raftery, M. A., Attardi, G.: Electrofocusing analysis of HeLa cell metaphase chromosomes. Biochem.11, 1654–1663 (1972).

    Google Scholar 

  6. Rice, R. H., Horst, J.: Isoelectric focusing of viruses in polyacrylamide gels. Virology49, 602–604 (1972).

    Google Scholar 

  7. Spear, P. G., Roizman, B.: Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpes virion. J. Virol.9, 143–159 (1972).

    Google Scholar 

  8. Svensson, H.: Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH-gradients. III. Description of apparatus for electrolysis in columns stabilized by density gradients and direct determination of isoelectric points. Arch. Biochem. Biophys., Suppl.1, 132–138 (1962).

    Google Scholar 

  9. Vahlne, A. G., Blomberg, J.: Purification of herpes simplex virus. J. gen. Virol.22, 297–302 (1974).

    Google Scholar 

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Olofsson, S. Isoelectric focusing of herpes simplex virus. Archives of Virology 49, 93–98 (1975). https://doi.org/10.1007/BF01317529

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  • DOI: https://doi.org/10.1007/BF01317529

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