Summary
Trichoplusia ni cells infected with a low passage (LP) strain ofAutographa californica nuclear polyhedrosis virus (NPV) produce large numbers of polyhedral inclusion bodies (PIBs). Interference with PIB production occurs whenT. ni cells are first inoculated with a high passage (HP) strain and then challenged with the LP strain. PIB production is reduced 100 fold to the level seen with HP virus infection only.
This is a preview of subscription content, log in via an institution to check access.
References
Faulkner, P., Henderson, J. F.: Serial passage of a nuclear polyhedrosis disease virus of the cabbage looper(Trichopulsia ni) in a continuous tissue culture cell line. Virology50, 920–924 (1972).
Goodwin, R. H., Vaughn, J. L., Adams, J. R., Louloudes, S.: Replication of a nuclear polyhedrosis virus in an established insect cell line. J. Invertebr. Pathol.16, 284–288 (1970).
Goodwin, R. H., Vaughn, J. L., Adams, J. R., Louloudes, S. J.: The influence of insect cell lines and tissue culture media on Baculovirus polyhedra production. Misc. Publ. Ent. Soc. Amer. 66–72 (1973).
Hink, W. F.: Established insect cell line from the cabbage looper,Trichoplusia ni. Nature (London)226, 466–467 (1970).
Hink, W. F., Vail, P. V.: A plaque assay for titration of alfalfa looper nuclear polyhedrosis virus in a cabbage looper (TN-368) cell line. J. Invertebr. Pathol.22, 168–174 (1973).
Hink, W. F., Strauss, E.: Replication of alfalfa looper nuclear polyhedrosis virus in theTrichoplusia ni (TN-368) cell line. In:Kurstak, E., Maramorosch, K. (eds.), Invertebrate Tissue Culture: Applications in Medicine, Biology and Agriculture, 369–374. New York: Academic Press 1976.
Hink, W. F., Strauss, E.: Replication and passage of alfalfa looper nuclear polyhedrosis virus plaque variants in cloned cell cultures and larval stages of four host species. J. Invertebr. Pathol.27, 49–55 (1976).
Huang, A. S., Baltimore, D.: Defective viral particles and viral disease processes. Nature (London)226, 325–327 (1970).
Kimura, M., McIntosh, A. H.: Dual infection of theTrichoplusia ni cell line with theChilo iridescent virus (CIV) andAutographa californica nuclear polyhedrosis virus (NPV). In:Kurstak, E., Maramorosch, K. (eds.), Invertebrate Tissue Culture: Applications in Medicine, Biology and Agriculture, 391–394. New York: Academic Press 1976.
Mackinnon, E. A., Henderson, J. F., Stoltz, D. B., Faulkner, P.: Morphogenesis of nuclear polyhedrosis virus under conditions of prolonged passagein vitro. J. Ultrastruct. Res.49, 419–435 (1974).
McIntosh, A. H., Maramorosch, K., Rechtoris, C.: Adaptation of an insect cell line(Agallia constricta) in a mammalian cell culture medium.In Vitro 8, 375–378 (1972).
McIntosh, A. H., Padhi, S. B.:In vitro andin vivo comparative studies of several nuclear polyhedrosis viruses (NPVs) by neutralization, immunofluorescence and polyacrylamide gel electrophoresis (PAGE). In:Kurstak, E., Maramorosch, K. (eds.), Invertebrate Tissue Culture: Applications in Medicine, Biology and Agriculture, 331–338. New York: Academic Press 1976.
McIntosh, A. H., Rechtoris, C.: Insect cells: colony formation and cloning in agar medium.In Vitro 10, 1–5 (1974).
McIntosh, A. H., Maramorosch, K.: Retention of insect virus infectivity in mammalian cell cultures. J. N. Y. Ent. Soc.81, 175–182 (1973).
Reed, L. J., Muench, H.: A simple method of estimating fifty per cent end points. Amer. J. Hyg.27, 493–497 (1938).
Vail, P. V., Jay, D. L., Hink, W. F.: Replication and infectivity of the nuclear polyhedrosis virus of the alfalfa looper,Autographa californica, produced in cells grownin vitro. J. Invertebr. Pathol.22, 231–237 (1973).
Vaughn, J. L.: The production of viruses for insect control in large scale cultures of insect cells. In:Maramorosch, K. (ed.), Invertebrate Tissue Culture: Research Applications, 295–303. New York: Academic Press 1976.
Wildy, P.: Classification and nomenclature of viruses. In:Melnick, J. L. (ed.), Monographs in Virology, 1–81. Basel: S. Karger 1971.
Author information
Authors and Affiliations
Additional information
With 1 Figure
Rights and permissions
About this article
Cite this article
McIntosh, A.H., Shamy, R. & Ilsley, C. Interference with polyhedral inclusion body (PIB) production inTrichoplusia ni cells infected with a high passage strain ofAutographa californica nuclear polyhedrosis virus (NPV). Archives of Virology 60, 353–358 (1979). https://doi.org/10.1007/BF01317507
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01317507