Summary
African swine fever virus isolates were titrated in swine monocyte cultures established in microtitre trays. Although technically simpler and less laborious than conventional tube assays the microtitration assay was less sensitive, but for routine and comparative titrations offers distinct advantages.
References
Enjuanes, L., Carrascosa, A. L., Moreno, M. A., Vinuela, E.: Titration of African swine fever (ASF) virus. J. gen. Virol.32, 471–473 (1976).
Greig, A.: The use of a microtitration technique for the routine assay of African swine fever virus. Arch. Virol.47, 287–289 (1975).
Heuschele, W. P., Hess, W. R.: Diagnosis of African swine fever by immunofluorescence. Trop. Anim. Hlth Prod.5, 181–186 (1973).
Malmquist, W. A., Hay, D.: Hemadsorption and cytopathic effect produced by African swine fever virus in swine bone marrow and buffy coat cultures. Amer. J. vet. Res.21, 104–108 (1960).
Pan, I. C., Shimizu, M., Hess, W. R.: African swine fever: microplaque assay by an immunoperoxidase method. Amer. J. vet. Res.39, 491–497 (1978).
Parker, J., Plowright, W.: Plaque formation by African swine fever virus. Nature219, 524–525 (1968).
Tessler, J., Hess, W. R., Pan, I. C., Trautman, R.: Immunofluorescence plaque assay for African swine fever virus. Canad. J. comp. Med.38, 443–447 (1974).
Tubiash, H. S.: Quantity production of leukocyte cultures for use in haemadsorption tests with African swine fever virus. Amer. J. vet. Res.24, 381–384 (1963).
Reed, L. J., Muench, H.: A simple method of estimating fifty per cent end points. Amer. J. Hyg.27, 493–497 (1938).
Author information
Authors and Affiliations
Additional information
With 1 Figure
Rights and permissions
About this article
Cite this article
Wardley, R.C., Wilkinson, P.J. A microtechnique for the titration for african swine fever virus. Archives of Virology 64, 93–95 (1980). https://doi.org/10.1007/BF01317395
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01317395