Summary
This paper reports on the variability of structural polypeptides of 32 strains of Herpes simplex virus 2 (HSV-2) isolated from various locations in the United States and Italy. Most strains were passaged a limited number of times at low multiplicity outside the human host; a few strains were characterized by numerous passages at variable multiplicities in cell culture. The acrylamide gel electrophoresis of polypeptides from purified virions revealed very few differences. Considering these differences the HSV-2 strains analysed can be classified into 3 groups according to the variability of three polypeptides. The study of polypeptides in HSV-2 infected cells revealed more differences than those seen in the structural proteins. Comparisons of the structural proteins specified by HSV-2 and HSV-1 virions, revealed clear variant features of the electrophoretic profiles, especially when pH of main gel was 8.9.
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Brewer, J. M., Pesce, A. J., Ashworth, R. B.: Experimental techniques in biochemistry, 139. Englewood Cliffs, N.J.: Prentice-Hall 1974.
Buchman, T. G., Roizman, B., Nahmias, A. J.: Exogenous genital reinfection with herpes simplex virus 2 demonstrated by restriction endonuclease fingerprinting of viral DNA. (In press, 1978.)
Cassai, E., Sarmiento, M., Spear, P. G.: Comparison of the virion proteins specified by herpes simplex virus types 1 and 2. J. Virol.16, 1327–1331 (1975).
Davis, B. J.: Disc electrophoresis. II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci.121, 404–427 (1964).
Dimmock, N. J., Watson, D. H.: Proteins specified by influenza virus in infected cells: analysis by polyacrylamide gel electrophoresis of antigens not present in the virion particle. J. gen. Virol.5, 499–509 (1969).
Dulbecco, R., Vogt, M.: Plaque formation and isolation of pure lines with poliomyelitis viruses. J. exp. Med.99, 167–182 (1954).
Frenkel, N., Roizman, B., Cassai, E., Nahmias, A.: A DNA fragment of herpes simplex 2 and its transcription in human cervical cancer tissue. Proc. Nat. Acad. Sci. U.S.A.69, 3784–3789 (1972).
Gibson, W., Roizman, B.: Proteins specified by herpes simplex virus. VIII. Characterization and composition of multiple capsid forms of subtypes 1 and 2. J. Virol.10, 1044–1052 (1972).
Halliburton, I. W.: Biochemical comparison of type 1 and type 2 herpes simplex viruses. In:Biggo, P. M., de Thè, G., Payne, L. N. (eds.), Oncogenesis and Herpesviruses, 432–438. Lyon, France: International Agency for Research on Cancer 1972.
Hayward, G. S., Frenkel, N., Roizman, B.: The anatomy of herpes simplex virus DNA: strain difference and heterogeneity in the location of restriction endonuclease cleavage sites. Proc. Nat. Acad. Sci. U.S.A.72, 1768–1772 (1975).
Hayward, G. S., Jacob, R. J., Wadsworth, S. C., Roizman, B.: Anatomy of herpes simplex virus DNA: Evidence for four populations of molecules that differ in the relative orientations of their long and short segments. Proc. Nat. Acad. Sci. U.S.A.72, 4243–4247 (1975).
Heine, J. W., Honess, R. W., Cassai, E., Roizman, B.: Proteins specified by herpes simplex virus. XII. The virion polypeptides of type 1 strains. J. Virol.14, 640–651 (1974).
Honess, R. W., Roizman, B.: Proteins specified by herpes simplex virus. IX. Identification and relative molar rates of synthesis of structural and non structural herpes virus polypeptides in the infected cells. J. Virol.12, 1347–1365 (1973).
Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London)227, 680–684 (1970).
Melnick, J. L., Adam, E.: Epidemiological approaches to determining whether herpesvirus is the etiological agent of cervical cancer. Prog. exp. Tumor Res.21, 49–69 (1978).
Nahmias, A., Sawanabori, S.: The genital herpes-cervical cancer hypothesis—10 years later. Prog. exp. Tumor Res.21, 117–139 (1978).
Ornstein, L.: Disc electrophoresis. I. Background and theory. Ann. N.Y. Acad. Sci.121, 321–349 (1964).
Pereira, L., Cassai, E., Honess, R. W., Roizman, B., Terni, M., Nahmias, A.: Variability in the structural polypeptides of herpes simplex virus 1 strains: potential application in molecular epidemiology. Infect. Immun.13, 211–220 (1976).
Powell, K. L., Courtney, R. J.: Polypeptides synthesized in herpes simplex virus type 2-infected HEp-2 cells. Virology66, 217–228 (1975).
Roizman, B., Spear, P. G.: Preparation of herpes simplex virus of high titer. J. Virol.2, 83–84 (1968).
Sarmiento, M.: Herpes simplex virus: investigation of the organization and function of the virion envelope. Thesis for PH. D., The University of Chicago, Dept. of Microbiology, 1977.
Spear, P. G., Roizman, B.: Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpes virion. J. Virol.9, 143–159 (1972).
Strnad, B. C., Aurelian, L.: Proteins of herpesvirus type 2: I. Virion, nonvirion, and antigenic polypeptides in infected cells. Virology69, 438–452 (1976).
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Cassai, E., Luca, D.D., Manservigi, R. et al. Comparative analysis of the virion polypeptides specified by Herpes simplex virus type 2 strains. Archives of Virology 64, 35–45 (1980). https://doi.org/10.1007/BF01317389
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DOI: https://doi.org/10.1007/BF01317389