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Intracellular glycosylation of influenza hemagglutinin: The effect of glucosamine

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Summary

The intracellular glycosylation of influenza virus hemagglutinin has been studied in calf kidney primary cultures infected with influenza virus A/NWS (H0N1). Intermediate glycosylated forms were obtained by the addition of glucosamine to the culture medium; they were characterized after [35S]-methionine labelling by their precursor relationship, by immunoprecipitation and peptide mapping. The underglycosylated hemagglutinins were distributed in classes of discrete molecular weight indicating that glycosylation occurred in a stepwise manner. A total of 8 different bands, suggesting 7 glycosylation sites, was obtained under conditions of both complete and incomplete glycosylation.

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Authors and Affiliations

  1. Research Department, Pharmaceuticals Division, Ciba-Geigy Ltd., Basle, Switzerland

    M. A. Horisberger & C. de Staritzky

  2. Department of Virology, Institut Pasteur du Brabant, Brussels, Belgium

    J. Content

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  1. M. A. Horisberger
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  2. C. de Staritzky
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  3. J. Content
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Horisberger, M.A., de Staritzky, C. & Content, J. Intracellular glycosylation of influenza hemagglutinin: The effect of glucosamine. Archives of Virology 64, 9–16 (1980). https://doi.org/10.1007/BF01317386

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  • DOI: https://doi.org/10.1007/BF01317386

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