Skip to main content
Log in

Monoclonal antibodies against Chilo Iridescent Virus (iridovirus type 6)

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Mouse hybridomas producing antibodies against proteins of Chilo Iridescent Virus were established by fusions of X 63-Ag 8-653 myeloma cells with spleen cells from balb/c mice immunized with purified disrupted viral particles. Forty monoclonal antibodies to CIV proteins have been characterized. By ELISA, 4 categoires of monoclonal antibodies were defined according to their ability to react with some selected viral protein fractions used as antigen. In preliminary studies, the specificity of 2 monoclonal antibodies for some viral polypeptides was determined by immunoblotting.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Barray, S., Devauchelle, G.: Etude des polypeptides de structure du virus iridescent deChilo suppressalis (Iridovirus type 6). Can. J. Microbiol.25, 841–849 (1979).

    Google Scholar 

  2. Burnette, W. N.: “Western blotting”: Electrophoretic transfer of proteins from sodium Dodecyl Sulfate — polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. biochem.112, 195–203 (1981).

    Google Scholar 

  3. Cerutti, M., Devauchelle, G.: Cell fusion induced by an invertebrate virus. Arch. Virol.61, 149–155 (1979).

    Google Scholar 

  4. Cerutti, M., Devauchelle, G.: Inhibition of macromolecular synthesis in cells infected with an invertebrate virus (iridovirus type 6 or CIV). Arch. Virol.63, 297–303 (1981).

    Google Scholar 

  5. Cerutti, M., Devauchelle, G.: Isolation and reconstitution of Chilo Iridescent Virus membrane. Arch. Virol.74, 145–155 (1982).

    Google Scholar 

  6. Cerutti, M., Devauchelle, G.: Characterization and localization and localization of CIV polypeptides. Virol.145, 123–131 (1985).

    Google Scholar 

  7. Chinchar, V. G., Metzger, D. W., Granoff, A., Goorha, R.: Localization of Frog Virus 3 proteins using monoclonal antibodies. Virology137, 211–216 (1984).

    Google Scholar 

  8. Farara, T., Attias, J.: Alkaline protease activity associated with iridescent virus type 6, FEBS Lett.20, 351–355 (1983).

    Google Scholar 

  9. Goding, J. W.: Antibody production by hybridomas. J. Immunol. Meth.39, 285–308 (1980).

    Google Scholar 

  10. Guerillon, J., Barray, S., Devauchelle, G.: Crossed-immunoelectrophoretic characterization of Chilo Iridescent virus surface antigens. Arch. Virol.73, 161–170 (1982).

    Google Scholar 

  11. Engvall, E., Perlmann, P.: Enzyme linked immunosorbent assay; ELISA III quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-wated tubes. J. Immunol.109, 129–135 (1972).

    Google Scholar 

  12. Laemmli, K.: Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature (London)227, 680–685 (1970).

    Google Scholar 

  13. Lefrancois, L., Lyles, D. S.: The interaction of antibody with the major surface glycoprotein of vesicular stomatis virus. Virology121, 157–167 (1982).

    Google Scholar 

  14. Monnier, C., Devauchelle, G.: Enzyme activities associated with an invertebrate iridovirus: nucleotide phosphohydrolase activity associated with iridescent virus type 6 (CIV). J. Virol.19, 180–186 (1976).

    Google Scholar 

  15. Monnier, C., Devauchelle, G.: Enzyme activities associated with an invertebrate iridovirus: Protein kinase activity associated with iridescent virus type 6 (Chilo Iridescent Virus). J. Virol.35, 444–450 (1980).

    Google Scholar 

  16. Orange-Balange, N., Devauchelle, G.: Lipid composition of an iridovirus type 6 (CIV). Arch. Virol.73, 363–367 (1982).

    Google Scholar 

  17. Orvell, C., Grandien, M.: The effects of monoclonal antibodies on biologic activities of structural proteins of sendai virus. J. of Immunol.129, 2779–2787 (1982).

    Google Scholar 

  18. Stoltz, D. B.: The structure of icosahedral cytoplasmic deoxyriboviruses. J. Ultrastruc. Res.37, 219–239 (1971).

    Google Scholar 

  19. Towbin, H., Straehelin, T., Gordon, J.: Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. P.N.A.S. U.S.A.76, 4350–4354 (1979).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Orange, N., Devauchelle, G. Monoclonal antibodies against Chilo Iridescent Virus (iridovirus type 6). Archives of Virology 90, 349–353 (1986). https://doi.org/10.1007/BF01317383

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01317383

Keywords

Navigation