Summary
Five multiply enveloped European isolates ofMamestra brassicae nuclear polyhedrosis virus (Oxford, German, French, Dutch and Danish) were found to be very closely related serologically using the enzyme linked immunosorbent assay (ELISA) double antibody sandwich method and immunodiffusion. By SDS-polyacrylamide gel electrophoresis of viral proteins and restriction endonuclease analysis of DNA using seven enzymes there appeared to be two variants as the Oxford and German isolates were distinct from the other three. The German isolate was shown to be more susceptible to Nonidet P40 detergent treatment affecting some nucleocapsid structural polypeptides which also reduced antigenicity in gel immunodiffusion plates. In bioassays of polyhedra, the Dutch isolate showed a higher LD50 than the other viruses although this was not statistically significant.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brown, D. A., Bud, H. M., Kelly, D. C.: Biophysical properties of the structural components of a granulosis virus isolated from the cabbage white butterfly(Pieris brassicae). Virology81, 317–327 (1977).
Crook, N. E., Payne, C. C.: Comparison of three methods of ELISA for baculoviruses. J. gen. Virol.46, 29–37 (1980).
Evans, H. F.: Quantitative assessment of the relationships between dosage and response of the nuclear polyhedrosis virus ofMamestra brassicae J. Invertebr. Pathol.37, 101–109 (1981).
FAO/WHO 1973, “The Use of Viruses for the Control of insect Pests and Disease Vectors”. WHO Technical Report No. 531/FAO Agricultural Studies No. 91, Geneva.
Finney, D. J.: Probit Analysis, 3rd ed. London: Cambridge University Press 1971.
Harrap, K. A., Payne, C. C.:Robertson, J. S.: The properties of three baculoviruses from closely related hosts. Virology79, 14–31 (1977).
Kelly, D. C., Brown, D. A.: Biochemical and biophysical properties of aMamestra brassicae multiple enveloped nuclear polyhedrosis virus. Arch. Virol.66, 133–141 (1980).
Kelly, D. C., Brown, D. A., Robertson, J. S., Harrap, K. A.: Biochemical, biophysical and serological properties of two singly enveloped nuclear polyhedrosis viruses fromHeliothis armigera andH. zea. Microbiologica3, 319–331 (1980).
Kelly, D. C., Edwards, M. L., Robertson, J. S.: The use of enzyme-linked immunosorbent assay to detect and discriminate between small iridescent viruses. Ann. appl. Biol.90, 369–374 (1978).
Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London)227, 680–685 (1970).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951).
Miller, L. K., Dawes, K. P.: Restriction endonuclease analysis for the identification of baculovirus pesticides. Appl. Environ. Microbiol.35, 411–421 (1978).
Smith, G. E., Summers, M. D.: Restriction maps of fiveAutographa californica M-NPV variants,Trichoplusia ni M-NPV andGalleria mellonella M-NPV with the endonucleases Sma 1, Kpn 1, Sac 1, Xho 1 and ECoRI. J. Virol.30, 828–838 (1979).
Summers, M. D., Smith, G. E.: Baculovirus structural polypeptides. Virology84, 390–402 (1978).
van Regenmortel, M. H. V., Burckard, J.: Detection of a wide spectrum of Tobacco mosaic virus strains by indirect enzyme-linked immunosorbent assays (ELISA). Virology106, 327–334 (1980).
Vlak, J. M., Gröner, A.: Identification of two nuclear polyhedrosis viruses from the cabbage moth,Mamestra brassicae (Lepidotera: Noctuidae). J. Invert. Pathol.35, 269–278 (1980).
Author information
Authors and Affiliations
Additional information
With 5 Figures
Rights and permissions
About this article
Cite this article
Brown, D.A., Evans, H.F., Allen, C.J. et al. Biological and biochemical investigations on five European isolates ofMamestra brassicae nuclear polyhedrosis virus. Archives of Virology 69, 209–217 (1981). https://doi.org/10.1007/BF01317336
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01317336