Summary
The E1 nucleic acid sequence of rubella virus strain Judith (RJ) has been cloned into anE. coli expression vector LB03. The reactivity of the expressed unglycosylated antigen (E1J) was compared with its glycosylated counterpart in native virus (RJ) usind rabbit and human sera. Rabbit antisera raised against RJ and E1J reacted differently with wild type, RJ (laboratory strain) and RA27/3 (vaccine virus) strains in a kinetic neutralisation test. Reciprocally, human post RA27/3 vaccination sera were also found to differ from post infection or post re-infection sera in their reactivity with RJ and E1J antigens. Our observations suggest that E1, in the conformation adopted in the RA27/3 virion may have unique antigenic properties.
References
Clarke DM, Loo TW, Hui I, Chong P, Gillam S (1987) Nucleotide sequence and in vitro expression of rubella virus 24S subgenomic mRNA encoding the structural proteins E1, E2 and C. Nucleic Acids Res 15: 3041–3057
Cusi MG, Rossolini GM, Cellesi C, Valensin PE (1988) Antibody response to wild rubella virus structural proteins following immunization with RA27/3 live attenuated vaccine. Arch Virol 101: 25–33
Enders G (1987) Prenatal diagnosis of intrauterine rubella. Eur J Clin Study Treat Infect 15: 162–164
Gould JJ, Butler M (1980) Differentiation of rubella virus strains by neutralisation kinetics. J Gen Virol 49: 423–426
Ho L (1983) A study of rubella virus antigens. MD thesis, University of London, London
Hobman TC, Shukin R, Gillam S (1988) Translocation of rubella virus glycoprotein E1 into the endoplasmic reticulum. J Virol 62: 4259–4264
Horstmann DM, Liebhaber H, Le Bouvier GL, Rosenberg DA, Halstead SB (1970) Rubella: reinfection of vaccinated and naturally immune persons exposed in an epidemic. N Engl J Med 15: 771–778
Ho-Terry L, Cohen A (1980) Degradation of rubella virus envelope components. Arch Virol 65: 1–13
Ho-Terry L, Londesborough P, Cohen A (1986) Analysis of rubella virus complement fixing antigens by polyacrylamide gel electrophoresis. Arch Virol 87: 219–218
Ho-Terry L, Terry GM, Londesborough P, Rees KR, Wielaard F, Denissen A (1988) Diagnosis of fetal rubella infection by nucleic acid hybridization. J Med Virol 24: 175–182
Iuorio JL, Hosking CS, Pyman C (1984) Retrospective diagnosis of congenital rubella. Br Med J 289: 1566–1568
Maniatis T, Fritsch EF, Sambrook J (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York
Mazancourt A, Waxham MN, Nicolas JC, Wolinsky JS (1986) Antibody response to the rubella virus structural proteins in infants with the congenital rubella syndrome. J Med Virol 19: 111–122
Nakhasi ML, Thomas D, Zheng D, Liu T-Y (1989) Nucleotide sequence and capsid, E2 and E1 protein genes of rubella virus vaccine strain RA27/3. Nucleic Acids Res 17: 4393–4394
Oker-Blom C, Pettersson RF, Summers MD (1989) Baculovirus polyhedrin promoter-directed expression of rubella virus envelope glycoproteins, E1 and E2, inSpodoptera frugiperda cells. Virology 172: 82–91
Terry GM, Ho-Terry L, Londesborough P, Rees KR (1989) A bio-engineered rubella E1 antigen. Arch Virol 104: 63–75
Vaheri A, Salonen E-M (1980) Evaluation of solid phase enzyme-immunoassay procedure in immunity surveys and diagnosis of rubella. J Med Virol 5: 171–181
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Londesborough, P., Terry, G. & Ho-Terry, L. Reactivity of a recombinant rubella E1 antigen expressed inE. coli . Archives of Virology 122, 391–397 (1992). https://doi.org/10.1007/BF01317201
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01317201