Summary
Thegag gene of a Japanese feline immunodeficiency virus (FIV) isolate, designated as FIV TM 2, was expressed inEscherichia coli as a fusion protein with TrpE. Using this expressed protein, an enzyme-linked immunosorbent assay was developed for detection of antibodies to FIVgag protein in feline sera. With serum samples from a cat experimentally infected with FIV, it was demonstrated that the period of seroconversion detected by this method corresponded to that by Western blotting.
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Furuya, T., Hasegawa, A., Saitoh, M. et al. Expression of feline immunodeficiency virusgag gene inEscherichia coli . Archives of Virology 122, 383–390 (1992). https://doi.org/10.1007/BF01317200
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DOI: https://doi.org/10.1007/BF01317200