Summary
Aminoterminal deletion mutants of the gene encoding the large hepatitis B surface protein were expressed in COS cells using a new expression vector. The truncated protein showed the same intracellular retention like the wild type protein. The findings show that the secretion block of the protein is not due to its aminoterminal myristylation.
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References
Bruss V, Ganem D (1991) The role of envelope proteins in hepatitis B virus assembly. Proc Natl Acad Sci USA 88: 1059–1063
Bruss V, Ganem D (1991) Mutational analysis of hepatitis B surface antigen particle assembly and secretion. J Virol 65: 3813–3820
Charnay P, Gervais M, Louise A, Galibert F, Tiollais P (1980) Biosynthesis of hepatitis B surface protein inEscherichia coli. Nature 286: 893–895
Chisari F, Filippi P, Buras J, MacLachan A, Popper H, Pinkert C, Palmiter R, Brinster R (1987) Structural and pathological effects of synthesis of hepatitis B virus large envelope polypeptide in transgenic mice. Proc Natl Acad Sci USA 84: 6909–6913
Heermann KH, Goldmann U, Schwartz W, Seyffarth T, Baumgarten H, Gerlich WH (1984) Large surface proteins of hepatitis B virus containing the pre-S sequence. J Virol 52: 396–402
Kuroki K, Russnak R, Ganem D (1989) Novel N-terminal amino acid sequence required for retention of a hepatitis B virus glycoprotein in the endoplasmic reticulum. Mol Cell Biol 9: 4459–4466
Marquardt O, Heermann KH, Seifer M, Gerlich WH (1987) Cell specific expression of pre-S 1 antigen and secretion of hepatitis B surface antigen. Arch Virol 96: 249–256
Mulligan RC, Berg P (1980) Expression of a bacterial gene in mammalian cells. Science 209: 1422–1427
Neurath AR, Kent SBH, Strick N, Parker N (1986) Identification and chemical synthesis of a host cell receptor binding site on hepatitis B virus. Cell 46: 429–436
Neurath AR, Strick N, Kent SBH, Parker K, Seto B, Girard M (1988) Design of synthetic peptides mimicking the immunologic and biologic functions of the pre-S1 sequence of the hepatitis B virus envelope protein. In: Ginsberg H, Brown F, Lerner RA, Chanock RM (eds) New chemical and genetic approaches to vaccination. Cold Spring Harbor Laboratory, New York, pp 229–234
Persing DH, Varmus HE, Ganem D (1986) Inhibition of secretion of hepatitis B surface antigen by a related presurface polypeptide. Science 234: 1388–1392
Persing DH, Varmus HE, Ganem D (1987) The pre-S 1 protein of hepatitis B virus is acylated at its amino terminus with myristic acid. J Virol 61: 1672–1677
Pontisso P, Ruvoletto MG, Gerlich WH, Heermann KH, Bardini R, Alberti A (1989) Identification of an attachment site for human liver plasma membranes on hepatitis virus B particles. Virology 173: 522–530
Prange R, Clemen A, Streeck RE (1991) Myristilation is involved in intracellular retention of hepatitis B virus envelope proteins. J Virol 65: 3919–3923
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, New York
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Korec, E., Gerlich, W.H. Expression of large hepatitis B envelope protein mutants using a new expression vector. Archives of Virology 122, 367–371 (1992). https://doi.org/10.1007/BF01317198
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DOI: https://doi.org/10.1007/BF01317198