Summary
In CFLP mice, intravenously administered partially purified interferon-α (IFN-α 2×106 µ/mg protein), prepared from human leukocytes, reduced carrageenan-induced paw swelling and produced slight irritation when injected into the footpad. Anti-human IFN-α serum abolished the anti-inflammatory effect but did not influence local phlogogenic activity. Highly purified human IFN-α (1.2×108 µ/mg protein) was also found to be anti-inflammatory after intravenous administration, and devoid of irritative effect at the injection site. These results suggest that human IFN-α possesses a direct inhibitory effect on acute inflammation in mice, and the irritation appearing at the site of its application might be due to some impurities being present in the partially purified preparations.
References
di Rosa, M., Giroud, J. P., Willoughby, D. A.: Studies of the mediators of the acute inflammatory response induced in rats in different sites by carrageenin and turpentine. J. Pathol.104, 15–29 (1971).
Fisher, P. B., Hermo, H., jr., Pringoli, D. R., Weinstein, I. B., Pestka, S.: Hybrid recombinant leukocyte interferon inhibits differentiation in murine B-16 melanoma cells. Biochem. Biophys. Res. Comm.119, 108–115 (1984).
Koltai, M., Mécs, I.: Inhibition of the acute inflammatory response by interferon inducers. Nature242, 525–526 (1973).
Koltai, M., Mécs, I., Kása, M.: On the anti-inflammatory effect of Sendai virus inoculation. Arch. Virol.67, 91–95 (1981).
Levy, L.: Carrageenan paw edema in the mouse. Life Sci.8, 601–606 (1969).
Levy-Koenig, R. E., Golgher, R. R., Pauker, K.: Immunology of interferons. II. Heterospecific activities of human interferons and their neutralization by antibody. J. Immunol.104, 791–796 (1970).
Mécs, I., Chin, D., Fox, F., Krim, M.: Purification of human leukocyte interferon by carboxymethyl-controlled pore glass bead chromatography. Arch. Virol.81, 303–311 (1984).
Mécs, I., Fox, F., Chin, D., Krim, M.: Preparation and use of immune affinity columns for the purification of human interferon alpha. (To be published.)
Smith, J. W., Sanford, J. P.: Viral arthritis. Ann. Intern. Med.67, 651–659 (1967).
Tóth, M., Mécs, I., Tóth, S., Erdész, V., Béládi, I.: Semi-crude human leucocyte interferon production in a simple medium. Acta Microbiol. Hung.3, 61–67 (1984).
Yaron, M., Yaron, I., Gurari-Rothman, D.: Stimulation of prostaglandin E production in cultured human fibroblasts by Poly (I). Poly (C) and human interferon. Nature267, 457–459 (1976).
Yaron, M., Baratz, M., Yaron, I., Zor, U.: Acute induction of joint inflammation in the rat by poly I · poly C. Inflammation3, 243–251 (1976).
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Mécs, I., Koltai, M. Direct evidence for the anti-inflammatory effect of human interferon-α in CFLP mice. Archives of Virology 85, 151–155 (1985). https://doi.org/10.1007/BF01317015
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DOI: https://doi.org/10.1007/BF01317015