Skip to main content
Log in

Isolation of large and small plaque variants of theAutographa California nuclear polyhedrosis virus

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Variants of theAutographa californica nuclear polyhedrosis virus which produce large (1.4 mm) or small (0.5 mm) plaques onSpodoptera littoralis cells have been isolated. Yields of extracellular virus and polyhedra by the large plaque variant were six-fold higher and 140-fold lower, respectively, than those obtained with the small plaque variant. However the two variants could not be distinguished whenSpodoptera frugiperda cells were used.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Brown, M., Faulkner, P.: Plaque assay of nuclear polyhedrosis viruses in cell culture. Appl. Environ. Microbiol.36, 31–35 (1978).

    Google Scholar 

  2. Fraser, M. J., Hink, W. F.: The isolation and characterization of the MP and FP plaque variants ofGalleria mellonella nuclear polyhedrosis virus. Virology117, 366–378 (1982).

    Google Scholar 

  3. Granados, R. R.: Infection and replication of insect pathogenic viruses in tissue culture. Advan. Virus Res.20, 189–236 (1976).

    Google Scholar 

  4. Harrap, K. A., Longworth, J. F.: An evaluation of purification methods for baculoviruses. J. Invertebr. Pathol.24, 55–62 (1974).

    Google Scholar 

  5. Harrap, K. A., Payne, C. C.: The structural properties and identification of insect viruses. Advan. Virus Res.25, 273–355 (1979).

    Google Scholar 

  6. Hink, W. F., Vail, P. V.: A plaque assay for titration of alfalfa looper nuclear polyhedrosis virus in a cabbage looper (TN-368) cell line. J. Invertebr. Pathol.22, 168–174 (1973).

    Google Scholar 

  7. Kelly, D. C.: Review article: Baculovirus Replication. J. gen. Virol.63, 1–13 (1982).

    Google Scholar 

  8. Lee, H. H., Miller, L. K.: Isolation, complementation and initial characterization of temperature-sensitive mutants of the baculovirusAutographa californica nuclear polyhedrosis virus. J. Virol.31, 240–252 (1979).

    Google Scholar 

  9. Potter, K. N., Faulkner, P., MacKinnon, E. A.: Strain selection during serial passage ofTrichoplusia ni nuclear polyhedrosis virus. J. Virol.18, 1040–1050 (1976).

    Google Scholar 

  10. Roberts, P. L., Naser, W.: Replication ofAutographa californica NPV in aSpodoptera littoralis cell line. In: Abstracts of the Fifth International Congress of Virology, Strasbourg, 1981, 292.

  11. Roberts, P. L.: Infectivity assays for theAutographa californica nuclear polyhedrosis virus usingSpodoptera littoralis cells. J. Virol. Methods10, 1–10 (1985).

    Google Scholar 

  12. Smith, G. E., Summers, M. D.: Restriction maps of fiveAutographa californica MNPV variants,Trichoplusia ni MNPV, andGalleria mellonella MNPV DNAs with endonucleasesSmaI,KpnI,BamHI,SacI,XhoI andEcoRI. J. Virol.30, 828–838 (1979).

    Google Scholar 

  13. Stockdale, H., Gardiner, G. R.: The influence of the condition of cells and medium on production of polyhedra ofAutographa californica nuclear polyhedrosis virusin vitro. J. Invertebr. Pathol.30, 330–336 (1977).

    Google Scholar 

  14. Summers, M. D., Volkman, L. E., Hsieh, C.-H.: Immunoperoxidase detection of baculovirus antigens in insect cells. J. gen. Virol.40, 545–557 (1978).

    Google Scholar 

  15. Tinsley, T. W.: The potential of insect pathogenic viruses as pesticidal agents. Ann. Rev. Entomol.24, 63–87 (1979).

    Google Scholar 

  16. Tinsley, T. W., Harrap, K. A.: Viruses of invertebrates. In:Fraenkel-Conrat, H., Wagner, R. R. (eds.), Comprehensive Virology, Vol. 12, 1–101. London: Plenum Press 1978.

    Google Scholar 

  17. Vaughn, J. L., Goodwin, R. H., Tompkins, G. J., McCawley, P.: The establishment of two cell lines from the insectSpodoptera frugiperda (Lepidoptera: Noctuidae). In vitro13, 213–217 (1977).

    Google Scholar 

  18. Volkman, L. E., Summers, M. D.: Nuclear polyhedrosis virus detection: Relative capabilities of clones developed fromTrichoplusia ni ovarian cell line TN-368 to serve as indicator cells in a plaque assay. J. Virol.16, 1630–1637 (1975).

    Google Scholar 

  19. Wood, H. A.: An agar overlay plaque assay method forAutographa californica nuclear-polyhedrosis virus. J. Invertebr. Pathol.29, 304–307 (1977).

    Google Scholar 

  20. Wood, H. A.: Isolation and replication of an occlusion body-deficient mutant of theAutographa californica nuclear polyhedrosis virus. Virology105, 338–344 (1980).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

With 1 Figure

Rights and permissions

Reprints and permissions

About this article

Cite this article

Roberts, P.L. Isolation of large and small plaque variants of theAutographa California nuclear polyhedrosis virus. Archives of Virology 85, 123–128 (1985). https://doi.org/10.1007/BF01317011

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01317011

Keywords

Navigation