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Nucleotide sequence of the coat protein gene of pelargonium leaf curl virus and comparison of the deduced coat protein amino acid sequence with those of other tombusviruses

Archives of Virology volume 129, pages 349–356 (1993)Cite this article

Summary

The sequence of 1,787 nucleotides (nts) in the genomic RNA of pelargonium leaf curl virus (PLCV) was determined. It included the entire coat protein (cp) gene (nts 585 to 1,754), 558 nts of the 3′ end of the putative RNA polymerase gene, 26 nts of an intercistronic region between the two genes and 33 nts downstream of the stop codon of the cp gene. The cp gene was cloned into the expression vector pET 8c and expressed inE. coli. The deduced cp amino acid sequence of PLCV was compared with those of five other tombusviruses. The closer the degree of serological relatedness between two viruses, the more similarity was found in their cp amino acid sequences not only in the protruding domains, but also in their random and shell domains and in the arm regions. Nucleic acid hybridization tests, cp amino acid comparisons and serological tests all suggest the same order of sequence for the relationships in the tombusvirus group.

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Affiliations

  1. Institut für Biochemie und Pflanzenvirologie, Biologische Bundesanstalt für Land- und Forstwirtschaft, Braunschweig, Federal Republic of Germany

    Y. Li, S. Bachmann, E. Maiß, U. Commandeur, E. Breyel, U. Timpe & R. Koenig

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  1. Y. Li
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  2. S. Bachmann
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  3. E. Maiß
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  4. U. Commandeur
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  5. E. Breyel
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  6. U. Timpe
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  7. R. Koenig
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Li, Y., Bachmann, S., Maiß, E. et al. Nucleotide sequence of the coat protein gene of pelargonium leaf curl virus and comparison of the deduced coat protein amino acid sequence with those of other tombusviruses. Archives of Virology 129, 349–356 (1993). https://doi.org/10.1007/BF01316911

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  • DOI: https://doi.org/10.1007/BF01316911

Keywords

  • Coat Protein
  • Coat Protein Gene
  • Nucleic Acid Hybridization
  • Protein Amino Acid Sequence
  • Amino Acid Comparison