Summary
Truncated α 4 genes were introduced into BHK tk− cells along with the neomycin phosphotransferase gene, that confers resistance to the eukaryotic antibiotic G 418, driven by the HSV-1 β tk promoter (β tk−neor). Stably transformed cell lines were obtained and studied for the ability of the resident truncated α 4 genes to regulate the expression of the β tk−neor, and for the ability of the truncated α 4 polypeptides to localize to the nuclei of transformed cells. The results indicated that the domain(s) for β gene induction and for nuclear localization of the α 4 protein are located within the N-terminal 288 amino acids of the protein.
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Sivropoulou, A., Arsenakis, M. Mapping of the functional domains of the α 4 protein of herpes simplex virus 1. Archives of Virology 129, 317–325 (1993). https://doi.org/10.1007/BF01316907
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DOI: https://doi.org/10.1007/BF01316907