Summary
The cDNA nucleotide sequences of the infectious pancreatic necrosis virus (IPNV) serotype-specific epitope of the major structural protein VP 2 were determined for a Scottish strain (Sp serotype), and an IPNV field isolate from Shetland. Comparison of the sequence data indicated that the field isolate was of the Sp serotype. The Scottish Sp strain and the field isolate showed less than 3% difference in nucleotide sequence over this region compared to the Norwegian N 1 strain, and all three European viruses differed by approximately 23% from the Canadian Jasper strain.
References
Azad AA, Jagadish MN, Brown MA, Hudson PJ (1987) Deletion mapping and expression inEscherichia coli of the large genomic segment of a birnavirus. Virology 161: 145–152
Bayliss CD, Spies U, Shaw K, Peters RW, Papageorgiou A, Muller H, Boursnell MEG (1990) A comparison of the sequences of segment A of four infectious bursal disease virus strains and identification of a variable region in VP 2. J Gen Virol 71: 1303–1312
Becht H, Muller H, Muller HK (1988) Comparative studies on structural and antigenic properties of two serotypes of infectious bursal disease virus. J Gen Virol 69: 631–640
Brown F (1986) The classification and nomenclature of viruses: summary of results of the international committee on taxonomy of viruses in Sendai, September 1984. Intervirology 25: 141–143
Caswell-Reno P, Reno PW, Nicholson B (1986) Monoclonal antibodies to infectious pancreatic necrosis virus: analysis of viral epitopes and comparison of different isolates. J Gen Virol 67: 2193–2205
Christie KE, Haverstein LS, Djupvik HO, Ness S, Endresen C (1988) Characterisation of a new serotype of infectious pancreatic necrosis virus isolated from Atlantic salmon. Arch Virol 103: 167–177
Dabre P (1988) Introduction to practical molecular biology. Wiley, Chichester
Davies RW (1985) DNA sequencing. In: Rickwood D, Hames BD (eds) Gel electrophoresis of nucleic acids. IRL Press, Oxford, pp 117–172
Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acid Res 12: 387–395
Diseases of Fish Act (1937); amended as Diseases of Fish Bill (1983). HMSO, London
Duncan R, Dobos P (1986) The nucleotide sequence of infectious pancreatic necrosis virus (IPNV) dsRNA segment A reveals one large ORF encoding a precursor polyprotein. Nucleic Acid Res 14: 5934
Haverstein LS, Kalland KH, Christie KE, Endresen C (1991) Sequence of the large double-stranded RNA segment of the N 1 strain of infectious pancreatic necrosis virus: a comparison with otherBirnaviridae. J Gen Virol 71: 299–308
Hill BJ (1982) Infectious pancreatic necrosis virus and its virulence. In: Roberts RJ (ed) Microbial diseases of fish. Academic Press, London, pp 91–114
MacDonald RD, Gower DA (1981) Genomic and phenotypic divergence among three serotypes of aquatic birnaviruses (infectious pancreatic necrosis virus). Virology 114: 187–195
Okamoto N, Sano T, Hedrick RP, Fryer JL (1983) Antigenic relationships of selected strains of infectious pancreatic necrosis virus and European eel virus. J Fish Dis 6: 19–25
Sanger F, Nicklem S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467
Snyder DB, Lana DP, Cho BR, Marquardt WW (1988) Group and strain-specific neutralisation sites of IBDV defined with monoclonal antibodies. Avian Dis 32: 527–534
Somogyi P, Dobos P (1980) Virus-specific RNA synthesis in cells infected by infectious pancreatic necrosis virus. J Virol 33: 129–139
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Pryde, A., Melvin, W.T. & Munro, A.L.S. Nucleotide sequence analysis of the serotype-specific epitope of infectious pancreatic necrosis virus. Archives of Virology 129, 287–293 (1993). https://doi.org/10.1007/BF01316903
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DOI: https://doi.org/10.1007/BF01316903