Summary
The early extra- and intra-cellular interaction between adenovirus type 2 and HeLa cells was studied in the presence of benzyl alcohol as a fluidizing agent. The processes of virus attachment and internalization were not affected at 5–15 mM of benzyl alcohol at 25°C. Under the same conditions an enhancement by 45% at the most was demonstrated for the cell-mediated virion uncoating. By completely blocking virion internalization with 50 mM azide the uncoating was reduced to 20% of the normal level. The remaining surfacelocated uncoating was not affected by benzyl alcohol. It was demonstrated that an enhancement of the intracellular virion uncoating was followed by a raised production of the hexon antigen, which was interpreted as an increase in the specific infectivity of the virus.
References
Belin M-T, Boulanger P (1987) Processing of vimentin occurs during the early stages of adenovirus infection. J Virol 6: 2559–2566
Bramhall J, Wisnieski B (1981) The role of lipids in virus-cell interaction. In: Lonberg-Holm K, Philipson L (eds) Receptors and recognition, ser B, vol 8. Chapman and Hall, London, pp 141–153
Carriere B, Le Grimellec C (1986) Effects of benzyl alcohol on enzyme activities and D-glucose transport in kidney brush-border membranes. Biochim Biophys Acta 857: 131–138
Ciechanover A, Schwartz AL, Dautry-Varsat A, Lodish HF (1983) Kinetics of internalization and recycling of transferrin and the transferrin receptor in a human hepatoma cell line. J Biol Chem 258: 9681–9689
Colley CM, Metcalfe JC (1972) The localisation of small molecules in lipid bilayers. FEBS Lett 24: 241–246
Everitt E, Ekstrand H, Boberg B, Hartley-Asp B (1990) Estramustine phosphate reversibly inhibits an early stage during adenovirus replication. Arch Virol 111: 15–28
Everitt S, Svensson U, Wohlfart C, Persson R (1986) Requirements for initial adenovirus uncoating and internalization. In: Crowell RL, Lonberg-Holm K (eds) Virus attachment and entry into cells. American Society for Microbiology, Washington, DC, pp 196–204
Fraker PJ, Speck JC Jr (1978) Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3 a,6 a-diphenylglycoluril. Biochem Biophys Res Commun 80: 849–857
Friedlander G, Le Grimellec C, Giocondi M-C, Amiel C (1987) Benzyl alcohol increases membrane fluiditiy and modulates cyclic AMP synthesis in intact renal epithelial cells. Biochim Biophys Acta 903: 341–348
Gordon LM, Sauerheber RD, Esgate JA, Dipple I, Marchmont RJ, Houslay MD (1980) The increase in bilayer fluidity of rat liver plasma membranes achieved by the local anesthetic benzyl alcohol affects the activity of intrinsic membrane enzymes. J Biol Chem 255: 4519–4527
Houslay MD, Gordon LM (1983) The activity of adenylate cyclase is regulated by the nature of its lipid environment. In: Kleinzeller A, Martin BR (eds) Current topics in membranes and transport, vol 18. Academic Press, New York, pp 179–231
Jähnig F, Bramhall J (1982) The origin of a break in Arrhenius plots of membrane processes. Biochim Biophys Acta 690: 310–313
Karin M, Mintz B (1981) Receptor-mediated endocytosis of transferrin in developmentally totipotent mouse teratocarcinoma stem cells. J Biol Chem 256: 3245–3252
Kuhry J-G, Duportail G, Bronner C, Laustriat G (1985) Plasma membrane fluidity measurements on whole living cells by fluorescence anisotropy of trimethylammonium-diphenylhexatriene. Biochim Biophys Acta 845: 60–67
Levanon A, Kohn A, Inbar M (1977) Increase in lipid fluidity of cellular membranes induced by adsorption of RNA and DNA virions. J Virol 22: 353–360
Li JK-K, Williams RE, Fox CF (1975) Effects of temperature and host lipid composition on the infection of cells by Newcastle disease virus. Biochem Biophys Res Commun 62: 470–477
Lonberg-Holm K, Philipson L (1969) Early events of virus-cell interaction in an adenovirus system. J Virol 4: 323–338
Patterson S, Russell WC (1983) Ultrastructural and immunofluorescence studies of early events in adenovirus-HeLa cell interactions. J Gen Virol 64: 1091–1099
Prendergast FG, Haugland RP, Callahan PJ (1981) 1-[4-(trimethylamino)phenyl]-6-phenylhexa-1,3,5-triene: synthesis, fluorescence properties, and use as a fluorescence probe of lipid bilayers. Biochemistry 20: 7333–7338
Sainte-Marie J, Vignes M, Vidal M, Philippot JR, Bienvenüe A (1990) Effects of benzyl alcohol on transferrin and low density lipoprotein receptor mediated endocytosis in leukemic guinea pig B lymphocytes. FEBS Lett 262: 13–16
Scatchard G (1949) The attractions of proteins for small molecules and ions. Ann NY Acad Sci 51: 660–672
Spector AA, Yorek MA (1985) Membrane lipid composition and cellular function. J Lipid Res 26: 1015–1035
Svensson U (1985) Role of vesicles during adenovirus 2 internalization into HeLa cells. J Virol 55: 442–449
Svensson U, Persson R (1984) Entry of adenovirus into HeLa cells. J Virol 51: 687–694
Svensson U, Persson R, Everitt E (1981) Virus-receptor interaction in the adenovirus system. I. Identification of virion attachment proteins of the HeLa cell plasma membrane. J Virol 38: 70–81
Varga MJ, Weibull C, Everitt E (1991) Infectious entry pathway of adenovirus type 2. J Virol 65: 6061–6070
Wohlfart CEG, Everitt E (1985) Assessment of specific virus infectivity and virus neutralization by a progeny virus immunotitration method as exemplified in an adenovirus system. J Virol Methods 11: 241–251
Yeagle PL (1989) Lipid regulation of cell membrane structure and function. FASEB J 3: 1833–1842
Yoshimura A, Kobayashi T, Hidaka K, Kuwano M, Ohnishi S-I (1987) Altered interaction between Sendai virus and a Chinese hamster cell mutant with defective cholesterol synthesis. Biochim Biophys Acta 904: 159–164
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Blixt, Y., Varga, M.J. & Everitt, E. Enhancement of intracellular uncoating of adenovirus in HeLa cells in the presence of benzyl alcohol as a membrane fluidizer. Archives of Virology 129, 265–277 (1993). https://doi.org/10.1007/BF01316901
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DOI: https://doi.org/10.1007/BF01316901