Summary
A series of monoclonal antibodies were developed against bovine rotavirus Q17. Among the five high affinity antibodies characterized, two (RQ 31 and RQ 4) were able to neutralize type G 6 viruses and may be specific for PB 1 type virus. Seventy seven feces from diarrheic calves were tested by “double sandwich” ELISA using four monoclonal and one polyclonal anti-rotavirus antibodies. The combination of mono- and polyclonal antibodies thus appears to be a more efficient strategy for detection and typing of bovine rotaviruses.
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Cornaglia, E., Elazhary, Y. & Talbot, B. Bovine rotavirus type detection by neutralizing monoclonal antibodies. Archives of Virology 129, 243–250 (1993). https://doi.org/10.1007/BF01316899
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DOI: https://doi.org/10.1007/BF01316899