Archives of Virology

, Volume 129, Issue 1–4, pp 77–92 | Cite as

Isolation and characterization of simian immunodeficiency virus from African white-crowned mangabey monkeys (Cercocebus torquatus lunulatus)

  • K. Tomonaga
  • J. Katahira
  • M. Fukasawa
  • M. A. Hassan
  • M. Kawamura
  • H. Akari
  • T. Miura
  • T. Goto
  • M. Nakai
  • M. Suleman
  • M. Isahakia
  • M. Hayami
Original Papers


Forty-eight of 236 sera from seven species of African non-human primates in Kenya, including those of white-crowned mangabey monkeys (Cercocebus torquatus lunulatus) had antibodies to simian immunodeficiency viruses (SIVs). Isolates of simian lentivirus were obtained from seropositive white-crowned mangabey monkeys which are indigenous in West Africa. This virus, designated as SIVWCM, appeared morphologically similar to HIV by electron microscopy, showed Mg2+-dependent reverse transcriptase activity, and induced cytopathic effects in human CD 4-positive cells. Western blotting analysis revealed thatenv products of SIVWCM cross-reacted with those of SIVAGM more strongly than with those of HIV-1 and SIVMAC, and clear hybridization bands were detected with an SIVAGM probe. For comparison of the virus sequence with those of other primate lentiviruses, part of thepol gene and the long terminal repeats (LTRs) were amplified and cloned. Sequencing showed that SIVWCM isolates were closely related to SIVAGM isolates. This study suggested that SIVAGM from theCercopithecus genus and SIVWCM from theCercocebus genus may be members of an SIV group that is genetically distinct from the SIV from a sooty mangabey monkey (SIVSMM) of the genusCercocebus, to which the white-crowned mangabey monkey also belongs.


Western Blotting Analysis Reverse Transcriptase Simian Immunodeficiency Virus Cytopathic Effect Terminal Repeat 
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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • K. Tomonaga
    • 1
  • J. Katahira
    • 1
  • M. Fukasawa
    • 1
  • M. A. Hassan
    • 1
    • 2
  • M. Kawamura
    • 1
  • H. Akari
    • 1
  • T. Miura
    • 1
  • T. Goto
    • 3
  • M. Nakai
    • 3
  • M. Suleman
    • 2
  • M. Isahakia
    • 2
  • M. Hayami
    • 1
  1. 1.Research Center for Immunodeficiency Virus, Institute for Virus ResearchKyoto UniversityKyotoJapan
  2. 2.Institute of Primate ResearchNational Museum of KenyaNairobiKenya
  3. 3.Department of MicrobiologyOsaka Medical CollegeTakatuki-shi OsakaJapan

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