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In vitro studies on bile acid deconjugation and lipolysis inhibition byGiardia lamblia

Digestive Diseases and Sciences volume 26, pages 700–704 (1981)Cite this article

Abstract

In vitro studies were conducted to determine whetherGiardia lamblia can deconjugate bile acids or inhibit lipolysis. CulturedG. lamblia trophozoites failed to deconjugate either glycine- or taurine-conjugated cholic acid, deoxycholic acid, or chenodeoxycholic acid. However, sonicated trophozoites significantly inhibited the hydrolysis of tributyrylglycerol by porcine pancreatic lipase. This inhibition was not dependent upon the presence of bile acids but was dependent upon the concentration ofG. lamblia sonicate. Heating the sonicate for 20 min at 60° C abolished the inhibitory effect; dialysis of the sonicate did not affect inhibition. We conclude that culturedG. lamblia trophozoites do not deconjugate bile acids and that sonicatedG. lamblia inhibit lipolysisin vitro.

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Affiliations

  1. recipient of a Veterans Administration Associate Investigator Award, No. 8007-101, cc 108, Department of Medicine (Division of Gastroenterology) of the Veterans Administration Medical Center, Denver, Colorado

    Phillip D. Smith MD, Charles R. Horsburgh Jr MD & William R. Brown MD

  2. recipient of a Veterans Administration Associate Investigator Award, No. 8007-101, cc 108, the University of Colorado Health Sciences Center, Denver, Colorado

    Phillip D. Smith MD, Charles R. Horsburgh Jr MD & William R. Brown MD

Authors
  1. Phillip D. Smith MD
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  2. Charles R. Horsburgh Jr MD
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  3. William R. Brown MD
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This study was supported in part by the Medical Research Service of the Veterans Administration.

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Smith, P.D., Horsburgh, C.R. & Brown, W.R. In vitro studies on bile acid deconjugation and lipolysis inhibition byGiardia lamblia . Digest Dis Sci 26, 700–704 (1981). https://doi.org/10.1007/BF01316858

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  • DOI: https://doi.org/10.1007/BF01316858

Keywords

  • Public Health
  • Hydrolysis
  • Lipase
  • Glycine
  • Bile Acid