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Bovine viral diarrhoea virus-specific bovine monoclonal antibody

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Summary

A bovine monoclonal antibody specific for bovine viral diarrhoea virus (BVDV) recognized all the reference strains of BVDV and over 90% of the field isolates tested. It neutralized the virus and precipitated a 56 k–58 k viral protein.

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References

  1. Bahnemann HG (1976) Inactivation of viruses in serum with binary ethyleneimine. J Clin Microbiol 3: 209–210

    Google Scholar 

  2. Bolin S, Moennig V, Kelso Gourley NE, Ridpath J (1988) Monoclonal antibodies with neutralizing activity segregate isolates of bovine viral diarrhea virus into groups. Arch Virol 99: 117–123

    Google Scholar 

  3. Chamberlain JP (1979) Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem 98: 132–135

    Google Scholar 

  4. Cooper PD (1967) The plaque assay of animal viruses. In: Maramorosch K, Koprowski H (ed) Methods in virology, vol 3. Academic Press, New York, pp 243–311

    Google Scholar 

  5. Done JT, Terlecki S, Richardson C, Harkness JW, Sands JJ, Patterson DSP, Sweasey D, Shaw IG, Winkler CE, Duffell SJ (1980) Bovine virus diarrhoea-mucosal disease virus: pathogenicity for the fetal calf following maternal infection. Vet Rec 106: 473–479

    Google Scholar 

  6. Donis RO, Dubovi EJ (1987) Characterization of bovine viral diarrhoea-mucosal disease virus specific proteins in bovine cells. J Gen Virol 68: 1597–1605

    Google Scholar 

  7. Donis RO, Dubovi EJ (1987) Glycoproteins of bovine viral diarrhoea-mucosal disease virus in infected bovine cells. J Gen Virol 68: 1597–1605

    Google Scholar 

  8. Donis RO, Corapi W, Dubovi EJ (1988) Neutralizing monoclonal antibodies to bovine viral diarrhoea virus bind to the 56 k to 58 k glycoprotein. J Gen Virol 69: 77–86

    Google Scholar 

  9. Dubovi EJ, Corapi W, Donis RO (1986) Probing the antigenic diversity of cytopathic and noncytopathic BVD virus with monoclonal antibodies. In: American Association of Veterinary Laboratory Diagnosticians 29th annual proceedings, 1986. American Association of Veterinary Laboratory Diagnosticians, Brookings, SD, pp 259–270

    Google Scholar 

  10. Grahn TC, Fahning ML, Zemjanis R (1984) Nature of early reproductive failure caused by bovine viral diarrhea virus. J Am Vet Med Assoc 185: 429–432

    Google Scholar 

  11. Groves DJ, Clayton J, Morris BA (1988) A bovine monoclonal antibody to oestrone/oestradiol prepared by a (murine × bovine) × bovine interspecies fusion. Vet Immunol Immunopathol 18: 95–101

    Google Scholar 

  12. Kahrs RF, Scoot FW, de Lahuta A (1970) Bovine viral diarrhea-mucosal disease, abortion, and congenital cerebellar hypoplasia in a dairy herd. J Am Vet Med Assoc 156: 851–857

    Google Scholar 

  13. Kelling CL, Kennedy JE, Rump KK, Partridge JE, Paul PS (1988) Genetic diversity of bovine viral diarrhea isolates. In: 69th Conference of Research Workers in Animal Disease, Chicago, Illinois, 1988, 55.

  14. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. Nature 227: 680–685

    Google Scholar 

  15. Lloyd KCK, Morris DD (1985) Bovine viral diarrhea in a newborn calf. J Am Vet Med Assoc 186: 600–601

    Google Scholar 

  16. Lopez A, Maxie MG, Ruhnke L, Savan M, Thomson RG (1986) Cellular inflammatory response in the lungs of calves exposed to bovine viral diarrhea virus,Mycoplasma bovis, andPasteurella haemolytica. Am J Vet Res 47: 1283–1286

    Google Scholar 

  17. Magar R, Minocha HC, Montpetit C, Carman PS, Lecomte J (1988) Typing of cytopathic and noncytopathic bovine viral diarrhea virus reference and Canadian field strains using a neutralizing monoclonal antibody. Can J Vet Res 52: 42–45

    Google Scholar 

  18. Nguyen C, Rouchouse J, Pouradiorduteil X (1989) Contamination of foetal bovine serum by bovine diarrhea virus (Bvd or MDV) and other related pestivirus. Adv Animal Cell Biol Tech Bioproc 1989: 65–67

    Google Scholar 

  19. Nielsen KH, Henning MD (1989) Bovine monoclonal antibody specific forBrucella abortus lipopolysaccharide. Vet Immunol Immunopathol 21: 363–372

    Google Scholar 

  20. Nowinski R, Berglund C, Lane J, Lostrom M, Bernstein I, Young W, Hakomori S-I (1980) Human monoclonal antibody against Forssman antigen. Science 210: 537–539

    Google Scholar 

  21. Olafson P, MacCallum AD, Fox FH (1946) An apparently new transmissible disease of cattle. Cornell Vet 36: 205–213

    Google Scholar 

  22. Onisk DV, Srikumaran S, Kelling CL (1989) A microplate immunofluorescence assay for the detection of monoclonal antibodies against viral antigens in cell culture. J Immunol Methods 125: 203–206

    Google Scholar 

  23. Peters W, Greiser-Wilke I, Moenning V, Liess B (1986) Preliminary serological characterization of bovine viral diarrhoea virus strains using monoclonal antibodies. Vet Microbiol 12: 195–200

    Google Scholar 

  24. Potgieter LND, McCracken MD, Hopkins FM, Walker RD, Guy JS (1984) Experimental production of bovine respiratory tract disease with bovine viral diarrhea virus. Am J Vet Res 45: 1582–1585

    Google Scholar 

  25. Raybould TJG, Crouch CF, McDougall LJ, Watts TC (1985) Bovine-murine hybridoma that secretes bovine monoclonal antibody of defined specificity. Am J Vet Res 46: 426–427

    Google Scholar 

  26. Roeder PL, Jeffrey M, Cranwell MP (1986) Pestivirus fetopathogenicity in cattle: changing sequelae with fetal maturation. Vet Rec 118: 44–48

    Google Scholar 

  27. Rossi CR, Bridgman CR, Kiesel GK (1980) Viral contamination of bovine fetal lung cultures and bovine fetal serum. Am J Vet Res 41: 1680–1681

    Google Scholar 

  28. Roth JA, Bolin SR, Frank DE (1986) Lymphocyte blastogenesis and neutrophil function in cattle persistently infected with bovine viral diarrhea virus. Am J Vet Res 47: 1139–1141

    Google Scholar 

  29. Shulman M, Wilde CD, Kohler G (1978) A better cell-line for making hybridomas secreting specific antibodies. Nature 276: 269–271

    Google Scholar 

  30. Srikumaran S, Guidry AJ, Goldsby RA (1983) Bovine-mouse hybridoma that secrete bovine immunoglobulin G1. Science 220: 522–524

    Google Scholar 

  31. Srikumaran S, Guidry AJ, Goldsby RA (1984) Production and characterization of monoclonal bovine immunoglobulins G1, G2 and M from bovine-murine hybridomas. Vet Immunol Immunopathol 5: 323–342

    Google Scholar 

  32. Srikumaran S, Goldsby RA, Guidry AJ, Hague B, Onisk DV, Srikumaran P (1987) Library of monoclonal bovine immunoglobulins and monoclonal antibodies to bovine immunoglobulins. Hybridoma 6: 527–530

    Google Scholar 

  33. Srikumaran S, Onisk DV, Zamb TJ, Osorio FA, Rice DN (1990) Bovine monoclonal antibodies specific for bovine herpesvirus 1 glycoprotein g III. Am J Vet Res 51: 543–545

    Google Scholar 

  34. Tucker EM, Dain AR, Clarke SW, Donker RA (1984) Specific bovine monoclonal antibody produced by a re-fused mouse/calf hybridoma. Hybridoma 3: 171–176

    Google Scholar 

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Authors and Affiliations

  1. Department of Veterinary Science, University of Nebraska, Lincoln, Nebraska, U.S.A.

    D. V. Onisk, S. Srikumaran, C. L. Kelling & M. L. Frey

Authors
  1. D. V. Onisk
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  2. S. Srikumaran
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  3. C. L. Kelling
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  4. M. L. Frey
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Onisk, D.V., Srikumaran, S., Kelling, C.L. et al. Bovine viral diarrhoea virus-specific bovine monoclonal antibody. Archives of Virology 121, 219–225 (1991). https://doi.org/10.1007/BF01316756

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  • DOI: https://doi.org/10.1007/BF01316756

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