Summary
Genomic relationships between turkey and bovine coronavirus (TCV and BCV), which are currently placed in distinct antigenic groups, were demonstrated by hybridization using specific cDNA probes. BCV-specific recombinant plasmid probes p 52, p 27, and p 247, holding inserts derived from (probably nonstructural) genes, and plasmids pN 17 and pN 9 holding the N and M gene, respectively, permitted the detection of isolates of both BCV and TCV with similar sensitivities. Similarly, probing supernatants of cell cultures infected with several isolates of TCV, using probes pN 17 and pM 78, respectively holding the N gene of BCV and TCV, resulted in equally intense detection signals. Only a slight detection of MHV-3, which is antigenically related to BCV, was observed, whereas the probes did not allow the detection of IBV, TGEV, and HCV-229E, which are placed in antigenic groups separate from those of BCV and TCV. Detection of TCV was improved by hybridization with BCV-specific single-stranded (ss) probes holding sequences of the N and M genes and synthesized by the polymerase chain reaction. Diagnosis of TCV in 134 clinical samples by hybridization was better with PCR-produced ss BCV-specific probes than with ds PCR-produced probes or a combination of six recombinant plasmid probes holding non-overlapping BCV-specific cDNA sequences. Detection signals were absent when probing clinical samples with32P-labelled pUC-DNA.
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References
Adams NR, Hofstad MS (1971) Isolation of transmissible enteritis agent of turkeys in avian embryos. Avian Dis 15: 426–433
Ambinder RF, Charache P, Staal S, Wright P, Forman M, Diane Hayward S, Hayward GS (1986) The vector homology problem in diagnostic nucleic acid hybridization in clinical specimens. J Clin Microbiol 24: 16–20
Binns MM, Boursnell MEG, Foulds IJ, Brown TDK (1985) The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus. J Virol Methods 11: 265–269
Boireau P, Crucière C, Laporte J (1990) Nucleotide sequence of the glycoprotein S-gene of bovine enteric coronavirus and comparison with the S proteins of two mouse hepatitis virus strains. J Gen Virol 71: 487–492
Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–255
Dea S, Marsolais G, Beaubien J, Ruppanner R (1986) Coronaviruses associated with outbreaks of transmissible enteritis (Bluecomb) of turkeys in Quebec: hemagglutination properties and cell cultivation. Avian Dis 30: 319–326
Dea S, Tijssen P (1989) Detection of turkey enteric coronavirus by enzyme-linked immunosorbent assay and differentiation from other coronaviruses. Am J Vet Res 50: 226–231
Dea S, Garzon S, Tijssen P (1989) Isolation and trypsin-enhanced propagation of turkey entric (Bluecomb) coronavirus in a continuous human rectal tumour (HRT-18) cell line. Am J Vet Res 50: 1310–1318
Dea S, Verbeek JA, Tijssen P (1990) Antigenic and genomic relationships among turkey and bovine coronaviruses. J Virol 64: 3112–3118
Diegutis PS, Keirnan E, Burnett L, Nightingale BN, Cossart YE (1986) False-positive results with hepatitis B virus DNA not-hybridization in hepatitis B surface antigen-negative specimens. J Clin Microbiol 23: 797–799
Gubler U, Hoffman BJ (1983) A simple and very efficient method for generating cDNA libraries. Gene 25: 263–269
Gyllensten UB, Erlich HA (1988) Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of theHLA-DQA locus. Proc Natl Acad Sci USA 85: 7652–7656
Hanahan D (1983) Studies on transformation ofEschericia coli with plasmids. J Mol Biol 166: 557–580
Laporte J, Bobulesco P, Rossi F (1980) Une lignée cellulaire particulièrement sensible à le replication du coronavirus entétique bovin: les cellules HRT-18. C R Acad Sci Paris 290 D: 623–626
Lapps W, Hogue BG, Brian DA (1987) Sequence analysis of the bovine coronavirus nucleocapsid and matrix protein. Virology 157: 47–57
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York
Panagraphy B, Naqi SA, Hull CF (1973) Isolation and characterization of viruses associated with transmissible enteritis (Bluecomb) of turkeys. Avian Dis 17: 430–438
Parker MD, Cox GJ, Deregt D, Fitzpatrick DR, Babiuk LA (1989) Cloning and in vitro expression of the gene for the E3 haemagglutinin glycoprotein of bovine coronavirus. J Gen Virol 70: 155–164
Patel BL, Deshmukh DR, Pomeroy KA (1975) Fluorescent antibody test for rapid diagnosis of coronaviral enteritis of turkeys (Bluecomb). Am J Vet Res 36: 1265–1267
Pedersen NC, Ward I, Mengeling WL (1978) Antigenic relationships of the feline infectious peritonitis virus to coronaviruses of other species. Arch Virol 58: 45–53
Patel BL, Pomeroy BS, Gonder E, Cronkite CE (1976) Indirect fluorescent antibody test for the diagnosis of coronaviral enteritis of turkeys (Bluecomb). Am J Vet Res 37: 1111–1112
Pomeroy BS, Sieburth JM (1954) Bluecomb disease of turkeys. In: Proceedings 90th Annual Meeting. Am Vet Med Assoc: 321–328
Pomeroy KA, Patel BC, Larsen CT, Pomeroy BS (1978) Combined immunofluorescence and transmission electron microscopic studies of sequential intestinal samples from turkey embryos and poults infected with turkey enteritis virus. Am J Vet Res 39: 1348–1358
Pomeroy BS (1984) Coronaviral enteritis of turkeys. In: Hofstad MS, Barnes HJ, Calnek BW, Reid WM, Yoder HW (eds) Diseases of poultry, 8th edn. Iowa State University Press, Ames, Iowa, pp 553–559
Rigby PW, Dieckmann CW, Rhodes M, Berg P (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113: 237–251
Ritchie AE, Deshmukh DR, Larsen CT, Pomeroy BS (1973) Electron microscopy of coronavirus-like particles characteristic of turkey Bluecomb disease. Avian Dis 17: 546–558
Roychoudhury R, Wu R (1980) Terminal transferase-catalysed addition of nucleotides to the 3′ termini of DNA. In: Grosman L, Moldave K (eds) Methods in enzymology, vol 65. Academic Press, New York, pp 43–62
Saif LF, Saif YM, Theil KW (1985) Enteric viruses in diarrheic poults. Avian Dis 29: 798–811
Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Highuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487–491
Schowalter DB, Sommer SS (1989) The generation of radiolabeled DNA and RNA probes with polymerase chain reaction. Anal Biochem 177: 90–94
Shockley LJ, Kapke PA, Lapps W, Brian DA, Potgieter LNW, Woods RH (1987) Diagnosis of porcine and bovine enteric coronavirus infections using cloned cDNA probes. J Clin Microbiol 25: 1591–1596
Tijssen P (1985) Practice and theory of enzyme immunoassays. Elsevier, Amsterdam (Laboratory techniques in biochemistry and molecular biology, vol 15)
Verbeek A, Tijssen P (1988) Biotinylated and radioactive cDNA probes in the detection by hybridization of bovine enteric coronavirus. Mol Cell Probes 2: 209–223
Verbeek A, Dea S, Tijssen P (1990) Detection of bovine enteric coronavirus in clinical specimens by hybridization with cDNA probes. Mol Cell Probes 4: 107–120
Verbeek A, Tijssen P (1990) Polymerase chain reaction for probe synthesis and for direct amplification in detection of bovine coronavirus. J Virol Methods 29: 243–256
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Verbeek, A., Dea, S. & Tijssen, P. Genomic relationship between turkey and bovine enteric coronaviruses identified by hybridization with BCV or TCV specific cDNA probes. Archives of Virology 121, 199–211 (1991). https://doi.org/10.1007/BF01316754
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DOI: https://doi.org/10.1007/BF01316754