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Interleukin-1 alpha gene-transcription in murine keratinocytes is inhibited by HSV-1 infection

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Summary

The effect of in vitro infection with herpes simplex virus 1 (HSV-1) on the Interleukin-1 (IL-1) activity of murine keratinocytes was investigated. IL-1α mRNA synthesis was measured by the Northern blot technique, and the IL-1 protein production was measured in terms of the ability of dialysed supernatants from cultures of uninfected and HSV-1 infected keratinocytes to enhance mitogen-induced murine thymocyte proliferation. IL-1α mRNA-synthesis in uninfected keratinocytes was detected 24 h and 48 h after isolation of the keratinocytes. IL-1 protein secretion by these keratinocytes was measureable at 18 h and reached a peak of 73 h, whereas intracellular and membrane-bound IL-1 protein production reached a maximum after 25 h. Keratinocytes, which had been cultured in vitro for 18 h, were infected with HSV-1 for 2 h and further cultured for an additional 4 h or 22 h before IL-1 measurements. A marked reduction of IL-1α gene-expression was noted 6 hours after HSV-1-infection of keratinocytes, and nearly total shut-off was detected after infection for 24 h. Reduced gene-expression was paralleled by a reduction in the IL-1 protein secretion from the HSV-1 infected keratinocytes.

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Authors and Affiliations

  1. Departments of Molecular Virology and Dermatology, Hebrew University — Hadassah Medical School, Jerusalem, Israel

    C. Enk, E. Sprecher & Y. Becker

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Enk, C., Sprecher, E. & Becker, Y. Interleukin-1 alpha gene-transcription in murine keratinocytes is inhibited by HSV-1 infection. Archives of Virology 121, 141–151 (1991). https://doi.org/10.1007/BF01316750

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  • DOI: https://doi.org/10.1007/BF01316750

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