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Establishment of a variant type of turkey herpesvirus which releases cell-free virus into the culture medium in large quantities

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Summary

A variant turkey herpesvirus (HVT/VT) which releases 105.0 plaqueforming units per 0.1 ml of cell-free virus into the culture medium was established from the prototype HVT FC 126 strain (HVT/WT). Many enveloped and naked virions of HVT/VT but only naked virions of HVT/WT were found in the culture medium. By theHindIII restriction cleavage patterns, the HVT/VT DNAs were almost identical to those of HVT/WT. The HVT/VT was replication-defective in chickens.

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Author notes

  1. S. Yachida

    Present address: Aburahi Laboratories, Shionogi Research Laboratories, Shionogi and Co., Ltd., Koka-cho, Shiga, Japan

Authors and Affiliations

  1. Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo

    S. Yachida, T. Kondo, H. Izawa & T. Mikami

  2. Department of Molecular Biology, Tokai University School of Medicine, Bohseidai, Isehara, Japan

    K. Hirai

Authors
  1. S. Yachida
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  2. T. Kondo
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  3. K. Hirai
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  4. H. Izawa
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  5. T. Mikami
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Yachida, S., Kondo, T., Hirai, K. et al. Establishment of a variant type of turkey herpesvirus which releases cell-free virus into the culture medium in large quantities. Archives of Virology 91, 183–192 (1986). https://doi.org/10.1007/BF01316738

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  • DOI: https://doi.org/10.1007/BF01316738

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