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Comparison of the primate alphaherpesviruses

I. Characterization of two Herpesviruses from spider monkeys and squirrel monkeys and viral polypeptides synthesized in infected cells

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Summary

Biological and biochemical properties of two neurotropic herpesviruses of New World monkeys—Herpesvirus saimiri type 1 (HVS-1) andHerpesvirus ateles type 1 (HVA-1)—were examined and compared. HVS-1 and HVA-1 both exhibited a time course of replication similar to another primate herpesvirus, SA 8. Both viruses grew rapidly and high titers of infectious virus were readily produced. HVS-1 and HVA-1 were also able to replicate efficiently in cell lines derived from a number of primate and non-primate species. Analysis of proteins synthesized in infected cells revealed the presence of over 30 virus-specific proteins ranging from <30,000 to over 200,000 daltons apparent molecular weight. Both viruses specified synthesis of a major capsid polypeptide of 148,000 daltons. Pulse labeling of cells during infection demonstrated temporal differences in the kinetics of synthesis of individual viral proteins and post-translational modification of a number of viral polypeptides. Glycosylated polypeptides synthesized in HVS-1 and HVA-1 infected cells were identified which ranged from approximately 49,000 to 120,000 daltons. Structural polypeptides of HVA-1 and HVS-1 virions were identified by SDS-PAGE analysis of purified virions. Taken together with clinical data on the diseases caused by these viruses, these studies indicate that HVS-1 and HVA-1 appear similar in many respects to both the human herpes simplex viruses and alphaherpesviruses of other primates.

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Authors and Affiliations

  1. Division of Pediatrics, City of Hope Medical Center, Duarte, California

    Siao-Wen Mou, Cheng-Hui Song & R. Eberle

  2. Virology and Immunology Department, Southwest Foundation for Biomedical Research, San Antonio, Texas, U.S.A.

    J. K. Hilliard

Authors
  1. Siao-Wen Mou
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  2. J. K. Hilliard
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  3. Cheng-Hui Song
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  4. R. Eberle
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Additional information

With 6 Figures

A preliminary report of these studies was presented at the Sixth International Congress of Virology, Sendai, Japan, 1984.

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Mou, SW., Hilliard, J.K., Song, CH. et al. Comparison of the primate alphaherpesviruses. Archives of Virology 91, 117–133 (1986). https://doi.org/10.1007/BF01316733

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  • DOI: https://doi.org/10.1007/BF01316733

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