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Characterisation of an avian influenza virus nucleoprotein expressed inE. coli and in insect cells

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Summary

The nucleoprotein (NP) gene from influenza virus A/Shearwater/Australia/72 has been expressed intracellularly in bothE. coli and insect cells.E. coli-derived NP was identified by Western blot analysis as a 56 kDa protein which co-migrates with virion-derived NP. This protein was purified by immunoaffinity chromatography and a nitrocellulose binding assay showed that NP formed complexes with positive- and negative-sense influenza neuraminidase RNA transcribed in vitro. ELISA and Western blot analysis revealed that recombinant NP of 56 kDa was produced in high yields in insect cells using a baculovirus vector. Immunofluorescence microscopy revealed that NP was localised to the nucleus of infected insect cells.

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Authors and Affiliations

  1. CSIRO Division of Biomolecular Engineering, Parkville, Victoria, Australia

    V. R. Harley, K. A. Mather, B. E. Power, J. L. McKimm-Breschkin & P. J. Hudson

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  1. V. R. Harley
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  2. K. A. Mather
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  3. B. E. Power
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  4. J. L. McKimm-Breschkin
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  5. P. J. Hudson
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Harley, V.R., Mather, K.A., Power, B.E. et al. Characterisation of an avian influenza virus nucleoprotein expressed inE. coli and in insect cells. Archives of Virology 113, 267–277 (1990). https://doi.org/10.1007/BF01316679

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  • DOI: https://doi.org/10.1007/BF01316679

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