Summary
IG 1, a temperate phage ofBacillus subtilis, is strongly induced from its lysogens by 6-(p-hydroxyphenylazo)-uracil, an azopyrimidine known as selectively inhibiting theB. subtilis DNA polymerase III. IG 1 phages originated either by induction or infection multiply, abundantly, in the presence of that azopyrimidine, in spite of the drastic decline of cell viability. Chloramphenicol completely suppresses the induction effect and also blocks the formation of spontaneously induced phage particles.
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Bazill GW, Gross JD (1973) Mutagenic DNA polymerase inB. subtilis. Nature (New Biol) 243: 241–243
Brown NC (1970) 6-(p-hydroxyphenylazo)-uracil: a selective inhibitor of host DNA replication in phage-infectedBacillus subtilis. Proc Natl Acad Sci USA 67: 1454–1461
Brown NC (1971) Inhibition of bacterial DNA replication by 6-(p-hydroxyphenylazo)-uracil: differential effect on repair and semi-conservative synthesis inBacillus subtilis. J Mol Biol 59: 1–6
Brown NC, Handschumacher RE (1966) Inhibition of the synthesis of deoxyribonucleic acid in bacteria by 6-(p-hydroxyphenylazo)-2,4-dihydroxypyrimidine. J Biol Chem 241: 3083–3089
Cardoso MC, de Lencastre H, Fernandes RM, Lopez MC, Archer LJ (1986) Restriction pattern analysis of the DNAs from temperate bacteriophages IG 1, IG 3 and IG 4 ofBacillus subtilis. Broteria Genetica 82: 145–157
Cozzarelli NR, Low RL (1973) Mutational alteration ofBacillus subtilis DNA polymerase III to hydroxyphenylazopyrimidine resistance: polymerase III is necessary for DNA replication. Biochem Biophys Res Commun 51: 151–157
Cregg JM, Ito J (1979) A physical map of the genome of temperate phage ϕ 3T. Gene 6: 199–219
Fernandes RM (1986) PhD Thesis. University of Lisbon, Lisbon, Portugal
Fernandes RM, de Lencastre H, Archer LJ (1986) Three new temperate phages ofBacillus subtilis. J Gen Microbiol 132: 661–668
Fernandes RM, de Lencastre H, Archer LJ (1989) Specialized transduction inBacillus subtilis by the phages IG 1, IG 3 and IG 4. Arch Virol 105: 137–140
Gass KB, Low RL, Cozzarelly NR (1973) Inhibition of a DNA polymerase fromBacillus subtilis by hydroxyphenylazopyrimidines. Proc Natl Acad Sci USA 70: 103–107
Lavi U, Nattenberg A, Ronen A, Marcus M (1974)Bacillus subtilis DNA polymerase III is required for the replication of the virulent bacteriophage ϕ e. J Virol 14: 1337–1342
Mackenzie JM, Neville MM, Wright GE, Brown NC (1973) Hydroxyphenylazopyrimidines: characterization of the active forms and their inhibitory action on a DNA polymerase fromBacillus subtilis. Proc Natl Acad Sci USA 70: 512–516
Rowley SD, Brown NC (1977)Bacillus subtilis DNA polymerase is required for the replication of DNA of bacteriophages SPP1 and 0105. J Virol 21: 493–496
Rutberg L, Armentrout RW, Jonasson J (1972) Unrelatedness of temperateBacillus subtilis bacteriophages SPO2 and ϕ 105. J Virol 9: 732–737
Saedi MS, Yoo S-K, Watabe K, Garvey KJ, Ito J (1987) Characterization of a deletion mutant of bacteriophage ϕ 29. Virology 158: 313–319
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Fernandes, R.M., de Lencastre, H. & Archer, L.J. Action of 6-(p-hydroxyphenylazo)-uracil on bacteriophage IG 1. Archives of Virology 113, 177–181 (1990). https://doi.org/10.1007/BF01316671
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DOI: https://doi.org/10.1007/BF01316671