Skip to main content
SpringerLink
Log in

Effect of colistin on reduction of biliary flow induced by endotoxin ofE. coli

  • Original Articles
  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

In order to evaluate whether an antiendotoxin agent can inhibit the action of endotoxin ofEscherichia coli on biliary flow, we used 18 models of isolated perfused pig liver divided into four groups: (A) five perfused livers (control), (B) five perfused livers with 2 mg/100 ml of endotoxin added, (C) five perfused livers with 2 mg/100 ml of endotoxin added and treated with 1,500,000 IU of colistin, and (D) three perfused livers with 1,500,000 IU of colistin with no endotoxin. The livers were isolated and perfused according to a technique previously described by our laboratory. The viability of the perfusions was controlled by means of the mitochondrial respiration test. Transaminase and LDH levels were measured in the perfusion circuit. A significant reduction of the biliary flow was found in the group with endotoxin ofEscherichia coli (P<0.002). There was no reduction of biliary flow after addition of 1,500,000 IU of colistin (P< 0.001). No significant changes were observed in the other parameters measured, and no increase of the bile flow was observed in the colistin group. From our results we conclude that colistin is an inhibitorin vitro of the diminished biliary flow induced by endotoxin ofEscherichia coli.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bernstein J, Brown AK: Sepsis and jaundice in early infancy. Pediatrics 29:873, 1962

    Google Scholar 

  2. Arthur AB, Wilson BDR: Urinary infection presenting with jaundice. Br Med J 1:539, 1967

    Google Scholar 

  3. Eley DF, Hargreaves T, Lamberth HP: Jaundice in severe infections. Br Med J 2:75, 1965

    Google Scholar 

  4. Miller DF, Irvind RW: Jaundice in acute appendicitis. Lancet 1:321, 1969

    Google Scholar 

  5. Jadis S, Weinbaum G, Ajl SJ (eds): Microbial Toxins, Vol 5, Bacterial Endotoxins. New York, Academic Press, 1971

    Google Scholar 

  6. Utili R, Abernathy CO, Zimmerman H: Cholestatic effects ofEscherichia coli endotoxin on the isolated perfused rat liver. Gastroenterology 70:243, 1976

    Google Scholar 

  7. Rodriguez Montes JA, Rossi Raviolo I, Escartín P, et al: Modelo experimental de perfusión de hígado de cerdo. Gastroenterol Hepatol 1:131, 1978

    Google Scholar 

  8. Castillo-Olivares JL, Gosalvez M, Azpeitia M, et al: Mitochondrial respiration and oxidative phosphorylation during hepatic preservation. J Surg Res 2:85, 1972

    Google Scholar 

  9. Rodriguez Montes HA, Rossi I, Gutierrez Moguera M, et al: Acción de la endotoxina del Escherichia coli sobre hígado de cerdo aislado. Rev Clin Esp 148:127, 1973

    Google Scholar 

  10. Escobedo MB, Bartom LL, Marshall RE, et al: The frequency of jaundice in neonatal bacteria infections. Clin Pediatr 63:121, 1963

    Google Scholar 

  11. Miller DJ, Keeton GR, Webber BL, et al: Jaundice in severe bacterial infections. Gastroenterology 71:94, 1976

    Google Scholar 

  12. Fahrlander H, Huber F, Gloor F: Intrahepatic retention of bile in severe bacterial infections. Gastroenterology 47:590, 1964

    Google Scholar 

  13. Vermillon SE, Gregg JA, Baggenstess AH, et al: Jaundice associated with bacteremia. Arch Intern Med 124:611, 1969

    Google Scholar 

  14. Monges A, Tronconia HC, Legre M, et al: L'ictere satellite des infections urinaires gravidiques. Med Dig 2:343, 1973

    Google Scholar 

  15. Ng SH, Ramstrom JR: Urinary tract infections presenting with jaundice. Arch Dis Child 46:173, 1971

    Google Scholar 

  16. Klatskin G: Hepatitis associated with systemic infection.In Diseases of the liver, L Schiff (ed). Philadelphia, Lippincott, 1975, pp 711–754.

    Google Scholar 

  17. Utili R, Abernathy CO, Zimmerman HJ: Effects ofSalmonella enteritis endotoxin on the excretory functions of the isolated perfused rat liver. Proc Soc Exp Biol Med 155:184, 1977

    Google Scholar 

  18. Boyer JL, Remo P: Properties of (Na+, K+)-activated ATPase in rat liver plasma membranes enriched in bile canaliculi. Biochim Biophys Acta 401:59, 1975

    Google Scholar 

  19. Utili R, Abernathy CO, Zimmerman HJ: Inhibition of Na+, K+-ATPase by endotoxin: A possible mechanism for endotoxin-induced cholestasis. J Infect Dis 136:583, 1977

    Google Scholar 

  20. Shands JM: Affinity of endotoxin for membranes. J Infect Dis 128:197, 1973

    Google Scholar 

  21. Utili R, Abernathy XO, Aron SA, et al:In vivo effects ofEscherichia coli endotoxin on sulphbromophthalein clearance in the guinea pig. Experientia 33:1631, 1978

    Google Scholar 

  22. Utili R, Abernathy CO, Zimmerman HJ: Endotoxin effect on the liver. Life Sci 20:533, 1977

    Google Scholar 

  23. Zimmerman HJ, Fang M, Utili R, et al: Jaundice due to bacterial infection. Gastroenterology 77:362, 1979

    Google Scholar 

  24. Rifkind D, Palmer JD: Neutralization of endotoxin in chick by antibiotics. J Bacteriology 92:815, 1966

    Google Scholar 

  25. Cooperstock MS: Inactivation of endotoxin by polymyxin B. Antmicrob Agents Chemother 6:422, 1974

    Google Scholar 

  26. Nolan JP, Leibowitz AI: Endotoxin and the liver III. Modification of acute carbon tetrachloride injury by polymyxin B, an anti-endotoxin. Gastroenterology 75:445, 1978

    Google Scholar 

  27. Lopes JE, Inniss WE: Electron microscopic effect of polymyxin B onEscherichia coli lipopolysaccharide. J Bacteriol 100:1128, 1969

    Google Scholar 

  28. Nolan JP: The role of endotoxin in liver injury. Gastroenterology 69:1356, 1975

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Gastroenterology, Clínica Puerta de Hierro, c/San Martín dePorres, 4, Madrid 35, Spain

    P. Escartín MD, J. A. Rodriguez-Montes MD, V. Cuervas-Mons MD, I. Rossi BS, J. Alvarez-Cienfuegos MB, P. Maganto BS & J. L. Castillo-Olivares MD

  2. Department of Experimental Surgery, Clínica Puerta de Hierro and Universidad Autónoma de Madrid, Madrid, Spain

    P. Escartín MD, J. A. Rodriguez-Montes MD, V. Cuervas-Mons MD, I. Rossi BS, J. Alvarez-Cienfuegos MB, P. Maganto BS & J. L. Castillo-Olivares MD

  3. Universidad Autónoma de Madrid, Madrid, Spain

    P. Escartín MD, J. A. Rodriguez-Montes MD, V. Cuervas-Mons MD, I. Rossi BS, J. Alvarez-Cienfuegos MB, P. Maganto BS & J. L. Castillo-Olivares MD

Authors
  1. P. Escartín MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. A. Rodriguez-Montes MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. Cuervas-Mons MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. Rossi BS
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Alvarez-Cienfuegos MB
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. P. Maganto BS
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J. L. Castillo-Olivares MD
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by a grant of the Instituto Nacional de la Salud (Spain).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Escartín, P., Rodriguez-Montes, J.A., Cuervas-Mons, V. et al. Effect of colistin on reduction of biliary flow induced by endotoxin ofE. coli . Digest Dis Sci 27, 875–879 (1982). https://doi.org/10.1007/BF01316569

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01316569

Keywords

Search

Navigation