Summary
We have applied an RNA: RNA hybridization test for the detection of human coronavirus 229E. This test is undergoing further development but already allows a diagnosis of HCV 229E infection within 48 hours.
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References
Riski H, Hovi T (1980) Coronavirus infections of man associated with diseases other than the common cold. J Med Virol 6: 259–265
Isaacs D, Flowers D, Clarke JR, Valman HB, MacNaughton MR (1983) Epidemiology of coronavirus respiratory infections. Arch Dis Child 58: 500–503
Gubler U, Hofmann BJ (1983) A simple and very efficient method for generating cDNA libraries. Gene 25: 263–269
Melton DA, Krieg PA, Rebagliati MR, Maniatis T, Zinn K, Green MR (1984) Efficient in-vitro synthesis of biologically active RNA and RNA hybridization probes from plasmid containing a bacteriophage T7 promoter. Nucleic Acids Res 12: 7035–7056
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Myint, S., Siddell, S. & Tyrrell, D. The use of nucleic acid hybridization to detect human coronaviruses. Archives of Virology 104, 335–337 (1989). https://doi.org/10.1007/BF01315554
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DOI: https://doi.org/10.1007/BF01315554