Summary
Two bovine coronavirus (BCV) strains from diarrheic calf faeces were adapted to grow in HRT 18 cells and compared in immunofluorescence (IF), haemagglutination inhibition (HAI) and neutralisation (NT) tests with three other strains of BCV and a human coronavirus (HCV) strain obtained from other laboratories. Polyclonal antisera against these 6 viruses were raised in rabbits. No significant differences between viruses were detected by IF. In the HAI test the HCV strain was distinguishable from the 5 BCV strains and serological variation between the BCV strains was shown. HCV could be distinguished by NT test, but all BCV isolates were similar. Two monoclonal antibodies prepared against one of the BCV strains distinguished HCV in all three assays, and detected varying relationships between BCV strains.
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Archetti I, Horsfall FL (1950) Persistent antigenic variation of influenza A viruses after incomplete neutralization in vivo with heterologous immune serum. J Exp Med 92: 441–462
Brain DA, Hogue B, Lapps W, Potts B, Kapke P (1983) Comparative structure of coronaviruses. In: Proceedings, Fourth International Symposium on Neonatal Diarrhea. VIDO, Saskatchewan, pp 100–115
Dea S, Roy RS, Begin ME (1980) Physicochemical and biological properties of neonatal calf diarrhoea coronaviruses isolated in Quebec and comparison with the Nebraska calf coronavirus. Am J Vet Res 41: 23–29
Dea S, Roy RS, Elazhary M (1982) Antigenic variations among calf diarrhoea coronaviruses by immunodiffusion and counterimmuno-electrophoresis. Ann Rech Vet 13: 351–356
Deregt D, Crouch CF, Sabara MI, Gilchrist J, Babiuk LA, Hudson GR (1983) Preliminary studies of a bovine coronavirus (BCV) antigen responsible for neutralization. In: Proceedings, Fourth International Symposium on Neonatal Diarrhea. VIDO, Saskatchewan, pp 117–132
Deregt D, Babiuk LA (1987) Monoclonal antibodies to bovine coronavirus: characteristics and topographical mapping of neutralizing-epitopes on the E2 and E3 glycoproteins. Virology 161: 410–420
Deregt D, Sabara M, Babiuk L (1987) Structural proteins of bovine coronavirus and their intracellular processing. J Gen Virol 68: 2863–2877
El-Ghorr AA, Snodgrass DR, Scott FMM (1988) Evaluation of an immunogold electron microscopy technique for detecting bovine coronavirus. J Virol Methods 19: 215–224
Gerna G, Passarani N, Battaglia M, Rondanelli EG (1985) Human enteric coronaviruses: antigenic relatedness to human coronavirus OC 43 and possible etiologic role in viral gastroenteritis. J Infect Dis 151: 796–803
Hajer I, Storz J (1979) Structural polypeptides of the enteropathogenic bovine coronavirus strain Ly-138. Arch Virol 59: 47–57
Hogue BG, King B, Brian DA (1984) Antigenic relationships among proteins of bovine coronavirus, human respiratory coronavirus OC43, and mouse hepatitis coronavirus A 59. J Virol 51: 384–388
Laporte J, Bobulesco P (1981) Polypeptide structure of bovine enteritic coronavirus: comparison between a wild strain purified from feces and HRT 18 cell adapted strain. Adv Exp Med Biol 142: 181–184
Mebus CA, Stair EL, Rhodes MB, Twiehaus MF (1973) Neonatal calf diarrhea: propagation, attenuation, and characteristics of a coronavirus-like agent. Am J Vet Res 34: 145–150
Reynolds DJ, Debney TG, Hall GA, Thomas LH, Parsons KR (1985) Studies on the relationship between coronaviruses from the intestinal and respiratory tracts of calves. Arch Virol 85: 71–83
Sato K, Inaba Y, Kurogi H, Takahashi E, Satado K, Omori T, Matumoto M (1977) Hemagglutination by calf diarrhea coronavirus. Vet Microbiol 2: 83–87
Vautherot JF (1981) Plaque assay for titration of bovine enteritic coronavirus. J Gen Virol 56: 451–455
Wege H, Siddell S, Ter Meulen V (1982) The biology and pathogenesis of coronaviruses. Curr Top Microbiol Immunol 99: 165–200
Wyatt RG, Greenberg HB, James WD, Pittman AL, Kalica AR, Flores J, Chanock RM, Kapikian AZ (1982) Definition of human rotavirus serotypes by plaque reduction assay. Infect Immun 37: 110–115
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El-Ghorr, A.A., Snodgrass, D.R., Scott, F.M.M. et al. A serological comparison of bovine coronavirus strains. Archives of Virology 104, 241–248 (1989). https://doi.org/10.1007/BF01315546
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DOI: https://doi.org/10.1007/BF01315546