Summary
A nonenveloped icosahedral virus measuring 60 (±5) nm in diameter has been isolated from pike fry(Esox lucius). The isolate is stable to heat, lipid solvent and acid. Iododeoxyuridine has no effect on its replication. Serologically, the virus is related to the major European strains of infectious pancreatic necrosis virus (IPN) Sp and Ab.
The isolate is neutralized by normal trout serum and is not pathogenic for trout fry or for young pike. A line of CHSE-214 cells persistently infected with the isolate was established and has been passed 30 times.
References
Cohen, J., Poinsard, A., Scherrer, R.: Physicochemical and morphological features of Infectious Pancreatic Necrosis Virus. J. gen. Virol.21, 485–498 (1973).
Dobos, P., Hallett, R., Kells, D. T. C., Sorensen, O., Rowe, D.: Biophysical studies of Infectious Pancreatic Necrosis Virus. J. Virol.22, 150–159 (1977).
Dorson, M., de Kinkelin, P., Torchy, C.: Virus de la Nécrose Pancréatique Infectieuse: Acquisition de la sensibilité an facteur neutralisant du sérum de truite après passages successifs en culture cellulaire. C. R. Acad. Sci. Paris281, 1435–1438 (1975).
Kelly, R. K., Loh, P. C.: Electron microscopical and biochemical characterization of Infectious Pancreatic Necrosis Virus. J. Virol.10, 824–834 (1972).
Moss, L. H., Gravell, M.: Ultrastructure and sequential development of Infectious Pancreatic Necrosis Virus. J. Virol.3, 52–58 (1969).
Sano, T.: Viral diseases of cultured fishes in Japan. Fish Pathology10, 221–226 (1976).
Sonstergard, R. A., McDermott, L. A.: Isolation of Infectious Pancreatic Necrosis Virus from white suckers(Catastomus commersoni). Nature236, 174–175 (1972).
Underwood, B. O., Smale, C. J., Brown, F., Hill, B. J.: Relationship of a virus fromTellina tenuis to Infectious Pancreatic Necrosis Virus. J. gen. Virol.36, 93–109 (1977).
Wolf, K., Snieszko, S., Dunbar, G., Pyle, E.: Virus nature of Infectious Pancreatic Necrosis in trout. Proc. Soc. exp. Biol. Med.104, 105–108 (1960).
Wolf, K., Quimby, M. C., Carlson, C. P., Bullock, G. C.: Infectious Pancreatic Necrosis: Selection of virusfree stock from a population of carrier trout. J. Fish. Res. Bd. Canada25, 383–391 (1968).
Wolf, K., Quimby, M. C.: Infectious Pancreatic Necrosis: Clinical and immune response of adult trout to inoculation with live virus. J. Fish. Res. Bd. Canada26, 2511–2516 (1969).
Wood, H. A.: Viruses with double stranded RNA genomes. J. gen. Virol.20, 61–85 (1973).
Yamamoto, T.: Frequency of detection and survival of Infectious Pancreatic Necrosis Virus in a carrier population of brook trout(Salvelinus fontinalis) in a lake. J. Fish. Res. Bd. Canada32, 568–570 (1975).
Author information
Authors and Affiliations
Additional information
With 1 Figure
Rights and permissions
About this article
Cite this article
Ahne, W. Isolation and characterization of infectious pancreatic necrosis virus from pike(Esox lucius) . Archives of Virology 58, 65–69 (1978). https://doi.org/10.1007/BF01315537
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01315537