Summary
Thein vitro virus yield of MHV3 reached 107 PFU/ml in mouse DBT cells infected with a virus suspension in HEPES-buffered medium containing DEAE-dextran. The virus titer was 106 PFU/ml in the presence of 10 µg actinomycin D/ml. MHV3 grown in DBT cells gave three peaks of density (1.10–1.14 g/cm3, 1.18–1.20 g/cm3, and 1.25–1.31 g/cm3) in sucrose gradients. All these peaks retained infectivity.
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Takayama, N., Kirn, A. In vitro growth characteristics and heterogeneity of mouse hepatitis virus type 3. Archives of Virology 58, 29–34 (1978). https://doi.org/10.1007/BF01315532
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DOI: https://doi.org/10.1007/BF01315532