Summary
Syrian hamster interferon, produced by benzo (a) pyrene-transformed embryo cells does not display an affinity for Zn++ chelate-agarose. However, the interferon binds to Cu++ chelate-agarose and can be displaced from the column by a falling pH-gradient. The chromatography of crude hamster interferon preparation on a tandem of columns: Zn++ chelate-agarose → Cu++ chelate-agarose results in a 25-fold purification of interferon.
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Bollin, E., Sulkowski, E. Metal chelate affinity chromatography of hamster interferon. Archives of Virology 58, 149–152 (1978). https://doi.org/10.1007/BF01315407
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DOI: https://doi.org/10.1007/BF01315407