Summary
A variant of Sindbis virus which is much more infectious for mouse cells than the standard virus has been examined for biochemical properties which might be responsible for this biological difference. The variant has a much enhanced ability to adsorb to mouse plasmacytoma (MOPC 315) cells, but when these cells were pretreated with heparin, they were able to adsorb the standard virus almost as well as the variant. This suggested that there was a surface charge difference between variant and standard virus. Differential elution of the viruses from hydroxyapatite and the results of isoelectric focusing of the virion glycoproteins substantiate this interpretation. Both viral glycoproteins E1 and E2 from the variant were more negatively charged than those of the standard virus but we were unable to find changes in tryptic peptides of the variant. Differences were found in stability of the two virus strains to heat and proteolytic enzymes.
Similar content being viewed by others
References
Allison, A. C., Valentine, R. C.: Virus particle adsorption. III. Adsorption of viruses by cell monolayers and effects of some variables on adsorption. Biochim. biophys. Acta40, 400–410 (1960).
Basilico, C., di Majorca, G.: Mutant of polyomavirus with impaired adsorption to BHK Cells. J. Virol.13, 931–934 (1974).
Bengtsson, S., Dinter, Z., Philipson, L.: Genetic Markers associated with virulence of foot and mouth disease virus Proc. Soc. exp. Biol. Med.113, 1019–1022 (1963).
Bengtsson, S., Philipson, L., Pearson, H., Laurent, T. C.: The basis for the interaction between attenuated poliovirus and polyanions. Virology24, 617–625 (1964).
Bose, H. R., Brundige, M. A., Carl, G. Z., Sagik, B. P.: Peptide composition of Sindbis virus variants. Arch. ges. Virusforsch.31, 207–214 (1970).
Bose, H. R., Carl, G. Z., Sagik, B. P.: Separation of Sindbis plaque-type variants by calcium phosphate chromatography. Arch. ges. Virusforsch.29, 83–89 (1970).
Bose, H. R., Carl, G. Z., Sagik, B. P.: Purification of two plaque variants of Sindbis virus. Arch. ges. Virusforsch.31, 200–206 (1970).
Burge, B. W., Pfefferkorn, E. R.: Isolation and characteristics of temperature-sensitive mutants of Sindbis virus. Virology30, 204–213 (1966).
Cambell, J. B., Colter, J. S.: Isolation of a series of Mengo virus variants differing in sensitivity to polyanions. Canad. J. Microbiol.13, 651–657 (1967).
Clarke, D. H., Casals, J.: Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Amer. J. trop. Med. Hyg.7, 561–573 (1958).
Dalrymple, J. M., Schlesinger, S., Russell, P. K.: Antigenic characterization of two Sindbis glycoproteins separated by isoelectric focusing. Virology69, 93–103 (1976).
Korant, B. D., Lonberg-Holm, K., Yin, F. H., Noble-Harvey, J.: Fractionation of biologically active and inactive populations of human rhinovirus Type 2. Virology63, 384–394 (1975).
Lomniczi, B.: Thermostability of Newcastle disease virus strains of different virulence. Arch. Virol.47, 249–255 (1975).
Mak, T. W., O'Callaghan, J., Colter, J. S.: Studies of the early events of Mengo encephalitis virus in mouse fibroblast cells. Virology42, 1087–1096 (1970).
Ozaki, Y., Kumagai, K.: Effect of polyions on Japanese encephalitis virus. Arch. ges. Virusforsch.39, 83–91 (1972).
Pederson, C. E., Jr., Barrera, C. R., Sagik, B. P.: Immunochemical studies of Sindbis virus variants. Arch. ges. Virusforsch.41, 28–39 (1973).
Schlesinger, S., Schlesinger, M. J.: Formation of Sindbis virus proteins: Identification of a precursor for one of the envelope proteins. J. Virol.10, 925–932 (1972).
Sefton, B. M., Keegstra, K.: Glycoproteins of Sindbis virus: Preliminary characterization of the oligosaccharides. J. Virol.14, 522–530 (1974).
Simpson, R. W., Hauser, R. E.: Effect of heat on virions of wild-type Sindbis virus and a thermostable mutant. Virology34, 361–364 (1968).
Symington, J., McCann, A. K., Schlesinger, M. J.: Infectious virus-antibody complexes of Sindbis virus. Infect. Immun.15, 720–725 (1977).
Symington, J., Schlesinger, M. J.: Isolation of a Sindbis virus variant by passage on mouse plasmacytoma cells. J. Virol.15, 1037–1041 (1975).
Takimoto, K. K., Liebhaber, H.: Virus polysaccharide interactions. II. Enhancement of plaque formation and the detection of variants of poliovirus with dextran sulfate. Virology17, 499–501 (1962).
Vaheri, A.: Heparin and related polyanionic substances as virus inhibitors. Acta Pathol. Microbiol. Scand. Suppl.171, 1–98 (1964).
Walder, R., Liprandi, F.: Kinetics of heat inactivation of Venezuelan equine encephalomyelitis virus. Arch. Virol.51, 307–317 (1976).
Wittmann, G., Dietzschold, B., Bauer, K.: Some investigations on the adjuvant mechanism of DEAE-Dextran. Arch. Virol.47, 225–235 (1975).
Author information
Authors and Affiliations
Additional information
With 3 Figures
Rights and permissions
About this article
Cite this article
Symington, J., Schlesinger, M.J. Characterization of a Sindbis virus variant with altered host range. Archives of Virology 58, 127–136 (1978). https://doi.org/10.1007/BF01315405
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01315405