Summary
Rat endothelial cells were productively infected with rat cytomegalovirusin vitro. A typical CMV-like cytopathic effect resulting in a lytic infection was observed. Intracytoplasmic and intranuclear virus and viral antigens were detected by fluorescence and electron microscopy. Receptors for the Fc region of IgG in the cytoplasm and on the cell membrane were observed. Infectious virus was released in the supernatant of the infected cell cultures.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Andrews, B. S., Theofilopoulos, A. N., Peters, C. J., Loskutoff, D. J., Brandt, N. E., Dixon, F. J.: Replication of Dengue and Junin viruses in cultured rabbit and human endothelial cells. Sug. Immun.20, 776–781 (1978).
Banji, A., Salisbury, R.: Cytomegalovirus and vasculitis. B. Med. J.1, 623–624 (1978).
Benditt, E. P., Barrett, T., McDougall, J. K.: Viruses in the etiology of atherosclerosis. Proc. Natl. Acad. Sci. U.S.A.80, 6386–6389 (1983).
Bruggeman, C. A., Meyer, H., Dormans, P. H. J., Debie, W. M. H., Grauls, G. E. L. M., van Boven, C. P. A.: Isolation of a cytomegalovirus-like agent from wild rats. Arch. Virol.734, 231–241 (1982).
Bruggeman, C. A., Meyer, H., Bosman, F., van Boven, C. P. A.: The biology of ratcytomegalovirus infection. Intervirology (in press) (1985).
Bruggeman, C. A., Reinders, R., Debie, W. H. M., Frederk, P. M., Engels, W., van Boven, C. P. A.: Demonstration of an IgG-Fc receptor in rat cytomegalovirus infected cells. Arch. Virol.81, 79–89 (1984).
Burch, G. E.: Viruses and arteriosclerosis. Am. Heart J.87, 407–412 (1974).
Cines, D. B., Lyss, A. P., Bina, M., Corkey, R., Kefalides, N. A., Friedman, H. M.: Fc and C3 receptors induced by herpes simplex virus on cultured human endothelial cells. J. Clin. Invest.69, 123–128 (1982).
Curtis, J. L., Egbert, B. M.: Cutaneous cytomegalovirus vasculitis: an unusual clinical presentation of a common opportunistic pathogen. Hum. Pathol.13, 1138–1141 (1982).
Fabricant, C. G., Fabricant, J., Litrenta, M. M., Minick, C. R.: Virus-induced atherosclerosis. J. Exp. Med.148, 335–340 (1978).
Friedman, H. M., Macarak, E. J., MacGregor, R. R., Wolfe, J., Kefalides, N. A.: Virusinfection of endothelial cells. J. Inf. Dis.143, 266–273 (1981).
Kasten, F. H.: Mammalian myocardial cells. In:Kruse, P. F., Patterson, M. K. (eds.), Tissue Culture. Methods and Applications, 77–78. New York: Academic Press 1973.
Kim, K. S., Sapienza, V. J., Chen, C. M. J., Wisniewski, K.: Production and characterization of monoclonal antibodies specific for a glycosylated polypeptide of human cytomegalovirus. J. Clin. Microbiol.18, 331–343 (1983).
Laurila, P., Virtanen, I., Wartiovaara, J., Stenman, S.: Fluorescent antibodies and lectins strain intracellular structures in fixed cells treated with nonionic detergent. J. Histochem. Cytochem.26, 251–257 (1978).
MacGregor, R. R., Friedman, H. J., Macarak, E. J., Kegalides, N. A.: Virus infection of endothelial cells increases granulocyte adherence. J. Clin. Invest.65, 1469–1477 (1980).
Maciag, T., Cerundolo, J., Ilsley, S., Kelley, P. R., Foriand, R.: An endothelial cell growth factor from bovine hypothalamus: Identification and partial characterization. Proc. Nat. Acad. Sci. U.S.A.76, 5674–5678 (1979).
Melnick, J. L., Dreesman, G. R., MacCollom, C. H., Petrie, B. L., Burek, J., DeBakey, M. E.: Cytomegalovirus antigen within human arterial smooth musle cells. Lanceti, 644–647 (1983).
Mims, C. A., Gould, J.: Infection of salivary glands, kidneys, adrenals, ovaries and epithelial by murine cytomegalovirus. J. Med. Microbiol.12, 113–122 (1979).
Minick, C. R., Fabricant, C. G., Fabricant, J., Litrenta, M. M.: Atheroarteriosclerosis induced by infection with a herpesvirus. Am. J. Pathol.96, 673–706 (1979).
Myerson, D., Hackman, R. C., Nelson, J. A., Ward, D. C., McDougall, J. K.: Widerspread presence of histologically occult cytomegalovirus. Hum. Pathol.15, 430–439 (1984).
Woolf, N.: Endothelial alterations and atherogenesis. In:Crawford, T. (ed.), Pathology of Atherosclerosis: Postgraduate Pathology Series, 261–285. London: Butterworth 1982.
Author information
Authors and Affiliations
Additional information
With 4 Figures
Rights and permissions
About this article
Cite this article
Bruggeman, C.A., Debie, W.M.H., Grauls, G. et al. Cytomegalovirus infection of rat endothelial cellsin vitro . Archives of Virology 87, 265–272 (1986). https://doi.org/10.1007/BF01315304
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01315304