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Aujeszky's disease in the guinea pig: Cellular and humoral responses following immunization

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Summary

The fatal disease caused by virulent ADV in guinea pigs was found to be identical to that seen in sheep and cattle. Intramuscular (i.m.) injection of an avirulent strain of ADV (Bartha) yielded better immunity to challenge after 3 weeks than did intranasal (i.n.) immunization, and this was reflected in differences in histopathological changes in the brain.

Serum antibodies active in antibody-dependent cell-mediated cytotoxicity (ADCC) were titrated using polymorphonuclear leukocytes as effector cells. ADCC correlated fairly well with virus neutralization and was a far more sensitive technique. There was good, but not complete, correlation between ADCC and protection. Lymphocyte responsiveness to virus antigensin vitro was assessed by3H-thymidine uptake and lymphokine tests. Lymphocyte stimulation and mitogenic factor responses were low grade but blood lymphocyte stimulation was more pronounced in the better-protected animals. Macrophage migration inhibition correlated neither with serum ADCC nor with protection, being equally demonstrable in the two immunized groups.

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References

  1. Ashworth, L. A. E., Ford, W. H.: A microplate culture method for assay of guinea pig mitogenic factor. J. immunol. Methods9, 47–57 (1975).

    Google Scholar 

  2. Ashworth, L. A. E., Ford, W. H.: Mitogenic factor as anin vitro correlate of delayed hypersensitivity in the guinea pig. Int. Archs. Allergy appl. Immunol.50, 583–592 (1976).

    Google Scholar 

  3. Ashworth, L. A. E., Lloyd, G., Baskerville, A.: Antibody-dependent cell-mediated cytotoxicity (ADCC) in Aujeszky's disease. Arch. Virol.59, 307–318 (1979).

    Google Scholar 

  4. Babuik, L. A., Rouse, B. T.: Interactions between effector cell activity and lymphokines: Implications for recovery from herpesvirus infections. Int. Archs. Allergy appl. Immunol.57, 62–73 (1978).

    Google Scholar 

  5. Baskerville, A., Lloyd, G.: Attempts to immunize monkeys against experimental infection with Herpesvirus suis. J. comp. Pathol.87, 591–596 (1977).

    Google Scholar 

  6. Baskerville, A., McCracken, R. M., McFerran, J. B.: The histopathology of experimental rhinitis in pigs produced by a strain of Aujeszky's disease virus. Res. vet. Sci.12, 323–326 (1971).

    Google Scholar 

  7. Baskerville, A., McFerran, J. B., Dow, C.: Aujeszky's disease in pigs. Vet. Bull.43, 465–480 (1973).

    Google Scholar 

  8. Ford, W. H., Ashworth, L. A. E., Inder, S.: Effect of the concentration of inducing agent on the output of lymphokines in the guinea pig. Eur. J. Immunol.6, 135–138 (1976).

    Google Scholar 

  9. McFerran, J. B., Dow, C.: Virus studies on experimental Aujeszky's disease in calves. J. comp. Pathol.74, 173–179 (1964).

    Google Scholar 

  10. McFerran, J. B., Dow, C.: The distribution of the virus of Aujeszky's disease in experimentally infected sheep. Res. vet. Sci.5, 143–148 (1964).

    Google Scholar 

  11. Rocklin, R. E., Macdermott, R. P., Chess, L., Schlossman, S. F., David, J. R.: Studies on mediator production by highly purified human T and B lymphocytes. J. exp. Med.140, 1303–1316 (1974).

    Google Scholar 

  12. Rosenberg, G. L., Farber, P. A., Notkins, A. L.,In vitro stimulation of sensitized lymphocytes by Herpes simplex virus and vaccinia virus. Proc. Nat. Acad. Sci. U.S.A.69, 756–760 (1972).

    Google Scholar 

  13. Rouse, B. T., Babiuk, L. A.: Host defense mechanisms against infectious bovine rhinotracheitis virus:in vitro stimulation of sensitized lymphocytes by virus antigen. Infect. Immun.10, 681–684 (1974).

    Google Scholar 

  14. Shope, R. E.: Experiments on the epidemiology of pseudorabies. 1. Mode of transmission of the disease in swine and their possible role in its spread to cattle. J. exp. Med.62, 85–99 (1935).

    Google Scholar 

  15. Thomson, G. R., Mumford, J. A.:In vitro stimulation of foal lymphocytes with equid herpesvirus 1. Res. vet. Sci.22, 347–352 (1977).

    Google Scholar 

  16. Wilton, J. M. A., Ivanyi, L., Lehner, T.: Cell-mediated immunity in Herpesvirus hominis infections. Brit. med. J.i, 723–726 (1972).

    Google Scholar 

  17. Wittman, G., Bartenbach, G., Jakubik, J.: Cell-mediated immunity in Aujeszky Disease virus infected pigs 1. Lymphocyte stimulation. Arch. Virol.50, 215–222 (1976).

    Google Scholar 

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Authors and Affiliations

  1. PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, England

    L. A. E. Ashworth, A. Baskerville & G. Lloyd

Authors
  1. L. A. E. Ashworth
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  2. A. Baskerville
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  3. G. Lloyd
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Additional information

This work was carried out while this establishment under its former title “Microbiological Research Establishment” was under the management of the Ministry of Defence.

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Ashworth, L.A.E., Baskerville, A. & Lloyd, G. Aujeszky's disease in the guinea pig: Cellular and humoral responses following immunization. Archives of Virology 63, 227–237 (1980). https://doi.org/10.1007/BF01315029

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  • DOI: https://doi.org/10.1007/BF01315029

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