Skip to main content
SpringerLink
Log in

Virion polypeptides of poxviruses

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Structural polypeptides from a number of poxviruses were analysed by SDS-polyacrylamide gel electrophoresis. The patterns obtained with orthopoxviruses were generally quite similar to one another, but variola, monkeypox, cowpox and vaccinia viruses could be distinguished by their profiles in the molecular weight (mol. wt.) region around 30,000 to 40,000; some additional variation was found amongst cowpox and vaccinia strains. Whitepox virus was shown to have structural polypeptides indistinguishable from those of variola virus.

The structural polypeptides of poxviruses belonging to other genera were different from those of the orthopoxvirus, except those of mol. wt. about 122,000 and 97,000, which were common to all viruses irrespective of genus. A polypeptide of mol. wt. about 25,000 was also observed in all cases, though its position varied slightly with the individual virus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Andrewes, C., Pereira, H. G., Wildy, P.: In: Viruses of Vertebrates, 4th ed., 356–389. London: Baillière, Tindall 1978.

    Google Scholar 

  2. Arita, I., Henderson, D. A.: Monkeypox and whitepox viruses in West and Central Africa. Bull. Wld. Hlth. Org.53, 347–353 (1976).

    Google Scholar 

  3. Arita, M., Tagaya, I.: Structural polypeptides of several strains of orthopoxvirus. Microbiol. Immunol.21, 343–346 (1977).

    Google Scholar 

  4. Baxby, D.: Smallpox-like viruses from camels in Iran. Lancetii, 1062–1065 (1972).

    Google Scholar 

  5. Baxby, D., Hill, B. J.: Characteristics of new poxvirus isolated from Indian buffaloes. Arch. ges. Virsuforsch.35, 70–79 (1971).

    Google Scholar 

  6. Downie, A. W.: A study of the lesions produced experimentally by cowpox virus. J. Pathol. Bacteriol.48, 361–379 (1939).

    Google Scholar 

  7. Dumbell, K. R., Bedson, H. S.: Adaptation of variola virus to rabbit. J. Pathol. Bacteriol.91, 459–465 (1966).

    Google Scholar 

  8. Esposito, J. L., Obijeski, J. F., Nakano, J. H.: The virion and soluble antigen proteins of variola, monkeypox and vaccinia viruses. J. med. Virol.1, 95–110 (1977).

    Google Scholar 

  9. Fenner, F.: The biological characters of several strains of vaccinia, cowpox and rabbitpox viruses. Virology5, 502–529 (1958).

    Google Scholar 

  10. Foster, S. O., Brink, E. W., Hutchins, D., Pfier, J. M., Lourie, B., Moser, C. R., Cummings, E. C., Kutey, O. E. K., Eke, R. E. A., Titus, J. B., Smith, E. A., Hicks, J. W., Foege, W. H.: Human monkeypox. Bull. Wld. Hlth. Org.46, 569–576 (1972).

    Google Scholar 

  11. Gispen, R.: Analysis of poxvirus antigens by means of double diffusion. A method for direct serological determination of cowpox. J. Immunol.74, 134–141 (1955).

    Google Scholar 

  12. Gispen, R., Brand-Saathof, B.: “White” poxvirus strains from monkeys. Bull. Wld. Hlth. Org.46, 585–592 (1972).

    Google Scholar 

  13. Gispen, R., Brand-Saathof, B.: Three specific antigens produced in vaccinia, variola and monkeypox infections. J. inf. Dis.129, 289–295 (1974).

    Google Scholar 

  14. Herrlich, S., Mayr, A., Mahnel, E., Munz, E.: Experimental studies on transformation of the variola virus into vaccinia virus. Arch. ges. Virusforsch.21, 579 to 599 (1963).

    Google Scholar 

  15. Ikeda, T.: On the establishment of the Ikeda strain of vaccinia virus. Nihon Iji-Shimpo No. 2470, 67–68 (1971). (Text in Japanese.)

  16. Joklik, W. K.: The purification of four strains of poxvirus. Virology18, 9–18 (1962).

    Google Scholar 

  17. Joklik, W. K.: The preparation and characterization of highly purified radioactively-labelled poxvirus. Biochim. biophys. Acta61, 290–301 (1962).

    Google Scholar 

  18. Kempe, C. H., Fulginiti, V., Minamitani, M., Shinfield, H.: Smallpox vaccination of eczema patients with a strain of attenuated live vaccinia (CV1-78). Pediatrics42, 980–985 (1968).

    Google Scholar 

  19. Laemmli, U. K.: Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature (London)227, 680–685 (1970).

    Google Scholar 

  20. Lourie, B., Bingham, P. G., Evans, H. H., Goster, S. O., Nakano, J. H., Herrman, K. L.: Human infection with monkeypox virus: Laboratory investigation on six cases in West Africa. Bull. Wld. Hlth. Org.46, 633–639 (1972).

    Google Scholar 

  21. Lourie, B., Nakano, J. H., Kemp, G. E., Setzer, H. W.: Isolation of poxvirus from an African rodent. J. inf. Dis.132, 677–681 (1972).

    Google Scholar 

  22. Marennikova, S. S., Seluchina, E. M., Mal'ceva, N. N., Cimiskjan, K. L., Macevic, G. R.: Isolation and properties of the causal agent of a new variola-like disease (monkeypox) in man. Bull. Wld. Hlth. Org.46, 599–611 (1972).

    Google Scholar 

  23. Obijeski, J. F., Palmer, E. L., Gafford, L., Randall, C. C.: Polyacrylamide gel electrophoresis of fowlpox and vaccinia proteins. Virology51, 512–516 (1973).

    Google Scholar 

  24. Oda, M.: Plaque-hemadsorption technique for the detection of nonhemagglutinating vaccinia virus. Virology23, 432–434 (1964).

    Google Scholar 

  25. Potel, K.: Kamelpocken. In:Röhrer, H. (ed.), Handbuch der Virusinfektion bei Tieren, Band II, Spezieller Teil I, 473–474. Jena: Gustav Fischer Verlag 1967.

    Google Scholar 

  26. Sarov, L., Joklik, W. K.: Studies on the nature and location of the capsid polypeptides of vaccinia viruses. Virology50, 579–592 (1972).

    Google Scholar 

  27. Sato, K.: Studies on the cultivation of variola pathogen in chick embryos. Jikken Igaku Zasshi23, 36–54 (1939). (Text in Japanese.)

    Google Scholar 

  28. Smallpox surveillance. Weekly Epidemiological Record (W.H.O.)54, 1–8 (1979).

  29. Stern, W., Dales, S.: Biogenesis of vaccinia: Isolation and characterization of surface component that elicits antibody suppressing infectivity and cell-cell fusion. Virology75, 232–241 (1976).

    Google Scholar 

  30. Tagaya, I., Kitamura, T., Sano, Y.: A new mutant of dermovaccinia. Nature (London)192, 381–382 (1961).

    Google Scholar 

  31. Tsuchiya, Y., Tagaya, I.: Isolation of a multinucleated giant cell-forming and hemagglutinin-negative variant of variola virus. Arch. ges. Virusforsch.39, 292 to 295 (1972).

    Google Scholar 

  32. Umeno, S., Muto, K.: On the natural cowpox. Saikingaku Zasshi No. 140, 409–419 (1907). (Text in Japanese.)

  33. von Magnus, P., Andersen, E. K., Petersen, K. B., Birch-Andersen, A.: A pox-like disease in cynomolgus monkeys. Acta Pathol. Microbiol. Scand.46, 156–176 (1959).

    Google Scholar 

  34. Weber, K., Osborn, M.: The reliability of molecular weight determinations by dodecyl sulfate polyacrylamide gel electrophoresis. J. biol. Chem.244, 4406 to 4412 (1969).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Enteroviruses, National Institute of Health, Tokyo, Japan

    M. Arita & I. Tagaya

Authors
  1. M. Arita
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. Tagaya
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

With 10 Figures

Rights and permissions

Reprints and permissions

About this article

Cite this article

Arita, M., Tagaya, I. Virion polypeptides of poxviruses. Archives of Virology 63, 209–225 (1980). https://doi.org/10.1007/BF01315028

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01315028

Keywords

Search

Navigation