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Non-occluded particles of nuclear polyhedrosis virus infectingGalleria mellonella L.: Titrationin vivo andin vitro

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Summary

Infectivity of non-occluded particles of nuclear polyhedrosis virus infectingGalleria mellonella L. was determined with bioassay techniquein vivo and with plaque assay methodin vitro using SCLd135 (Quiot) established cell culture. A plaque-forming virus unit corresponds to about 50 ID50 forGalleria larvae injected into hemocoel for non-cloned and 0.1–1.0 ID50 for a virus isolate cloned from an infective plaque.

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Authors and Affiliations

  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian S.S.R., Kiev, USSR

    S. V. Komissarenko, E. N. Zherebtsova, L. P. Sutugina & L. V. Primatchenko

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  1. S. V. Komissarenko
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  2. E. N. Zherebtsova
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  3. L. P. Sutugina
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  4. L. V. Primatchenko
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Komissarenko, S.V., Zherebtsova, E.N., Sutugina, L.P. et al. Non-occluded particles of nuclear polyhedrosis virus infectingGalleria mellonella L.: Titrationin vivo andin vitro . Archives of Virology 61, 347–349 (1979). https://doi.org/10.1007/BF01315023

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  • DOI: https://doi.org/10.1007/BF01315023

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