Summary
The kinetics of ultraviolet inactivation for two human rhinoviruses and poliovirus were compared. No major differences in the rates of ultraviolet inactivation were detectable. All viral preparations inactivated by ultraviolet irradiation induced neutralizing antibody in guinea pigs. In contrast, when guinea pigs were immunized with a heat inactivated rhinovirus preparation, little or no neutralizing antibody was elicited. Immune electron microscopy of the heated rhinovirus preparations revealed the presence of particles resembling empty capsids. These results suggest that rhinoviruses and enteroviruses are affected in a similar manner when subjected to ultraviolet or heat inactivation.
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References
Breindl, M.: The structure of heated poliovirus particles. J. gen. Virol.11, 147 to 156 (1971).
Cameron, K. R.: Ultraviolet irradiation of herpes simplex virus: action spectrum for the survival of infectivity in relation to the small-plaque effect. J. gen. Virol.18, 51–54 (1973).
Conant, R. M., Hamparian, V. V.: Rhinoviruses: basis for a numbering system. 1. HeLa cells for propagation and serologic procedures. J. Immunol.100, 107–113 (1968).
Conant, R. M., Somerson, N. L., Hamparian, V. V.: Plaque Formation by Rhinoviruses. Proc. Soc. exp. Biol. Med.128, 51–56 (1968).
Dimmock, N. J.: Differences between the thermal inactivation of picornaviruses at “high” and “low” temperatures. Virology31, 338–353 (1967).
Dimmock, N. J., Harris, W. J.: Heterogeneity of apparently empty poliovirus particles stained with phosphotungstic acid after heating of the virus at “low” temperature. Virology31, 715–719 (1967).
Doggett, J. E., Bynoe, M. L., Tyrrell, D. A. J.: Some attempts to produce an experimental vaccine with rhinoviruses. Brit. med. J.1, 34–36 (1963).
Hinuma, Y., Katagiri, S., Fukuda, M., Fukushi, K., Watanabe, Y.: Kinetic studies on the thermal degradation of purified poliovirus. Biken J.8, 143–153 (1965).
Hinuma, Y., Katagiri, S., Aikawa, S.: Immune response to H particles of poliovirus. Virology40, 773–776 (1970).
Hollaender, A., Oliphant, J. W.: The inactivating effect of monochromatic ultraviolet radiation on influenza virus. J. Bacteriol.48, 447–454 (1944).
Hughes, J. H., Chema, S., Lin, N., Conant, R. M., Hamparian, V. V.: Acid lability of rhinoviruses: loss of C and D antigenicity after treatment at pH 3.0. J. Immunol.112, 919–925 (1974).
Hughes, J. H., Gnau, J. M., Hilty, M. D., Chema, S., Ottolenghi, A. C., Hamparian, V. V.: Picornaviruses: rapid differentiation and identification by immune electron microscopy and immunodiffusion. J. med. Microb.10, 203–212 (1977).
Hummeler, K., Hamparian, V. V.: Studies on the complement-fixing antigens of poliomyelitis. I. Demonstration of type and group specific antigens in native and heated viral preparations. J. Immunol.81, 499–505 (1958).
Hummeler, K., Anderson, T. F., Brown, R. A.: Identification of poliovirus particles of different antigenicity by specific agglutination as seen in the electron microscope. Virology16, 84–90 (1962).
Jordan, L., Mayor, H. D.: Studies on the degradation of poliovirus by heat. Microbios9, 51–60 (1974).
Katagiri, S., Hinuma, Y., Ishida, N.: Biophysical properties of poliovirus particles irradiated with ultraviolet light. Virology32, 337–343 (1967).
Katagiri, S., Hinuma, Y.: Complement-fixing antibodies produced in guinea pigs immunized with purified N and H antigens of poliovirus. Tohoku J. exp. Med.88, 153–160 (1966).
Le Bouvier, G. L.: The modification of poliovirus antigens by heat and ultraviolet light. Lancet2, 1013–1016 (1955).
Le Bouvier, G. L.: The D → C change in poliovirus particles. Brit. J. exp. Pathol.40, 605–620 (1959).
Lonberg-Holm, K., Yin, F. H.: Antigenic determinants of infective and inactivated human rhinovirus type 2. J. Virol.12, 114–123 (1973).
McGregor, S., Mayor, H. D.: Internal components released from rhinovirus and poliovirus by heat. J. gen. Virol.10, 203–207 (1971).
Powell, H. M., Culbertson, C. G.: Short term heat stability of poliomyelitis vaccine. Proc. Soc. exp. Biol. Med.89, 490–492 (1955).
Rauth, A. M.: The physical state of viral nucleic acid and the sensitivity of viruses to ultraviolet light. Biophys. J.5, 257–273 (1965).
Rivers, T. M., Gates, F. L.: Ultra-violet light and vaccine virus. II. The effect of monochromatic ultra-violet light upon vaccine virus. J. exp. Med.47, 45–49 (1928).
Roizman, B., Mayer, M. M., Roane, P. R., Jr.: Immunochemical studies of poliovirus. IV. Alteration of the immunologic specificity of purified poliomyelitis virus by heat and ultraviolet light. J. Immunol.82, 19–25 (1959).
Schmidt, N. J., Lennette, E. H.: Modification of the homotypic specificity of poliomyelitis complement-fixing antigens by heat. J. exp. Med.104, 99–120 (1956).
Schmidt, N. J., Dennis, J., Frommhagen, L. H., Lennette, E. H.: Serologic reactivity of certain antigens obtained by fractionation of coxsackie viruses in cesium chloride density gradients. J. Immunol.90, 654–662 (1963).
Sime, E. H., Bedson, H. S.: A comparison of ultraviolet action spectra for vaccinia virus and T2 bacteriophage. J. gen. Virol.18, 55–60 (1973).
Strum, E., Gates, F. L., Murphy, J. B.: Properties of the causative agent of a chicken tumor. II. The inactivation of the tumor-producing agent by monochromatic ultra-violet light. J. exp. Med.55, 441–444 (1932).
Van Elsen, A. A., Boeye, A., Teuchy, H.: Formation of fibrillar structures from poliovirus by alkaline disruption and other treatments. Virol.36, 511–514 (1968).
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Hughes, J.H., Mitchell, M. & Hamparian, V.V. Rhinoviruses: Kinetics of ultraviolet inactivation and effects of UV and heat on immunogenicity. Archives of Virology 61, 313–319 (1979). https://doi.org/10.1007/BF01315018
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DOI: https://doi.org/10.1007/BF01315018