Summary
Experimental infection with HVJ (haemagglutinating virus of Japan—the Sendai strain of parainfluenza 1 virus) in mice was studied. Aerosol infection of newborn mice with the wild-type virus (HVJ-W) retarded the development of body weight and killed the animals within a few weeks. Large amounts of virus were isolated from both the lungs and the nasal turbinates of infected mice. In contrast, newborn mice exposed by inhalation to a temperature-sensitive(ts) mutant (HVJ-pB) derived from an HVJ carrier culture showed no clinical signs and grew equally well as mock-infected animals. No infectious virus could be recovered from the lungs although thets mutant grew to moderate titre in the nasal turbinates.
The prior inoculation of newborn mice with thets mutant virus induced a state of significant resistance to subsequent challenge with the virulent wild-type virus.
No replication of challenge virus in both lungs and nasal turbinates could be detected and the animals were protected a lethal infection. It is suggested that an avirulent temperature-sensitive mutant which has lost the capacity to replicate in the lower respiratory tract but is still capable of multiplying in the nasal turbinates may be a promising candidate for use in live vaccines especially against the infectious disease of the lower respiratory tract.
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Anderson, M. J., Bainbridge, D. R., Pattison, J. R., Heath, R. B.: Cell-mediated immunity to Sendai virus infection in mice. Infect. Immun.15, 239–244 (1977).
Blandford, G., Heath, R. B.: Studies on the immune response and pathogenesis of Sendai virus infection of mice. 2. The immunoglobulin class of plasma cells in the bronchial sub-mucosa. Immunology26, 667–671 (1974).
Craighead, J. E.: Growth of parainfluenza type 3 virus and interferon production in infant and adult mice. Brit. J. exp. Pathol.47, 235–241 (1966).
Fukumi, H., Mizutani, H., Takeuchi, Y., Tajima, Y., Imaizumi, K., Tanaka, T., Kaneko, J.: Studies on Sendai virus infection in laboratory mice. Japan J. med. Sci. Biol.15, 153–163 (1962).
Fukumi, H., Takeuchi, Y.: Vaccination against parainfluenza 1 virus (type muris) infection in order to eradicate this virus in colonies of laboratory animals. Develop. biol. Standard.28, 477–481 (1974).
Iida, T., Tajima, M., Murata, Y.: Transmission of maternal antibodies to Sendai virus in mice and its significance in enzootic infection. J. gen. Virol.18, 247–254 (1973).
Kimura, Y., Ito, Y., Shimokata, K., Nishiyama, Y., Nagata, I., Kitoh, J.: Temperature-sensitive virus derived from BHK cells persistently infected with HVJ (Sendai virus). J. Virol.15, 55–63 (1975).
Kimura, Y., Norrby, E., Nagata, I., Ito, Y., Shimokata, K., Nishiyama, Y.: Homologous interference induced by a temperature-sensitive mutant derived from an HVJ (Sendai virus) carrier culture. J. gen. Virol.33, 333–343 (1976).
Kimura, Y., Örvell, C., Norrby, E.: Characterization of the polypeptides synthesized in cells infected with a temperature-sensitive mutant derived from an HVJ (Sendai virus) carrier culture. Arch. Virol. (in press).
Murphy, B. R., Chalhub, E. G., Nusinoff, S. R., Chanock, R. M.: Temperaturesensitive mutants of influenza virus. 2. Attenuation ofts recombinants for man. J. inf. Dis.126, 170–178 (1972).
Nagata, I., Kimura, Y., Ito, Y., Tanaka, T.: Temperature-sensitive phenomenon of viral maturation observed in BHK cells persistently infected with HVJ. Virology49, 453–461 (1972).
Potash, L., Lees, R. S., Greenberger, J. L., Hoyrup, A., Denney, L. D., Chanock, R. M.: A mutant of parainfluenza type 1 virus with decreased capacity for growth at 38° C and 39° C. J. inf. Dis.121, 640–647 (1970).
Robinson, T. W. E., Cureton, R. J. R., Heath, R. B.: The effect of cyclophosphamide on Sendai virus infection of mice. J. med. Microbiol.2, 137–145 (1969).
Sawicki, L.: Influence of age of mice on the recovery from experimental Sendai virus infection. Nature (London)192, 1258–1259 (1961).
Schulman, J. L., Kilbourne, E. D.: Experimental transmission of influenza virus infection in mice. 1. The period of transmissibility. J. exp. Med.118, 257–266 (1963).
Sever, J. L.: Application of a microtechnique to viral serological investigations. J. Immunol.88, 320–329 (1962).
Sugita, K., Maru, M., Sato, K.: A sensitive plaque assay of Sendai virus in an established line of monkey kidney cells. Japan. J. Microbiol.18, 262–264 (1974).
Van Nunen, M. C. J., Van Der Veen, J.: Experimental infection with Sendai virus in mice. Arch. ges. Virusforsch.22, 388–397 (1968).
Wagner, R. R.: Pathogenicity and immunogenicity for mice of temperature-sensitive mutants of vesicular stomatitis virus. Infect. Immun.10, 309–315 (1974).
Wright, P. F., Woodend, W. G., Chanock, R. M.: Temperature-sensitive mutants of respiratory syncytial virus:In vivo studies in hamsters. J. inf. Dis.122, 501–512 (1970).
Wright, P. F., Mills, J., Chanock, R. M.: Evaluation of a temperature-sensitive mutant of respiratory syncytial virus in adults. J. inf. Dis.124, 505–511 (1971).
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Kimura, Y., Aoki, H., Shimokata, K. et al. Protection of mice against virulent virus infection by a temperature-sensitive mutant derived from an HVJ (Sendai virus) carrier culture. Archives of Virology 61, 297–304 (1979). https://doi.org/10.1007/BF01315016
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DOI: https://doi.org/10.1007/BF01315016