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Comparison of indirect hemagglutination and51Chromium release tests for detection of herpes simplex virus types 1 and 2 antibodies in patients with recurrent herpes infections

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Summary

Indirect hemagglutination and51Cr release tests (IHAT and 51-CRT respectively) were compared in patients with recurrent herpes simplex virus (HSV) infections from whom HSV-1 or HSV-2 was isolated. Both tests were equally sensitive and specific in detecting HSV antibodies. However, IHAT was more specific in detecting homologous HSV antibody response in patients with recurrent HSV-2 infections. Past infections with HSV-1 in the patients with dual infections were detected by determining HSV-type specific antibodies by inhibition of IHAT. Cross absorption studies showed that the antibody reactivity measured by the two tests was qualitatively and quantitatively different. Nevertheless, IHAT has been found to be more appropriate test for seroepidemiologic studies of HSV-2 infections because of its specificity, rapidity and less cost, whereas, 51-CRT appears to measure antibodies against recent and more predominant type of infecting HSV.

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References

  1. Back, A. F., Schmidt, N. J.: Indirect hemagglutinating antibody response toHerpes virus hominis types 1 and 2 in immunized laboratory animals and in natural infections of man. Appl. Microbiol.28, 392–399 (1974).

    Google Scholar 

  2. Back, A. F., Schmidt, N. J.: TypingHerpes virus hominis antibodies and isolates by inhibition of indirect hemagglutination reaction. Appl. Microbiol.28, 400–405 (1974).

    Google Scholar 

  3. Back, A. F., Schmidt, N. J.: Reactivity of envelope, capsid and soluble antigens ofHerpes virus hominis types 1 and 2 in the indirect hemagglutination test. Infect. Immun.10, 102–106 (1974).

    Google Scholar 

  4. Bernstein, M. T., Stewart, J. A.: Indirect hemagglutination test for detection of antibodies to cytomegalovirus. Appl. Microbiol.21, 84–89 (1971).

    Google Scholar 

  5. Bernstein, M. T., Stewart, J. A.: Method for typing antisera toHerpes virus hominis by indirect hemagglutination inhibition. Appl. Microbiol.21, 680–684 (1971).

    Google Scholar 

  6. Christenson, B.: Complement dependent cytotoxic antibodies in the course of cervical carcinoma. Int. J. Cancer20, 694–701 (1977).

    Google Scholar 

  7. Fucillo, D. A., Moder, F. L., Catalano, L. W., Vincent, M. M., Sever, J. L.:Herpes virus hominis types I and II: A specific micro indirect hemagglutination test. Proc. Soc. exp. Biol. Med.133, 735–739 (1970).

    Google Scholar 

  8. Gupta, P. C., Seth, P., Banerji, A. K., Gupta, P. K., Roy, S.: Herpes simplex encephalitis. Neurology (India).20, 136–141 (1972).

    Google Scholar 

  9. Huraux, J. M., Bogossian, M., Fortier, B., Bricout, F.: Sensibilité de la réaction d'hémagglutination passive appliqué au titrage des anticorps herpétiques. Path. Biol.24, 325–328 (1976).

    Google Scholar 

  10. Johnson, L. D., Fuccillo, D. A., Stalder, H., Oxman, M. A., Fraser, C. E. O., Madden, D. L.: Comparison of indirect hemagglutination and indirect immunofluorescence tests with microneutralization tests for detection of type-specificHerpes virus hominis antibody. J. clin. Microbiol.9, 384–390 (1979).

    Google Scholar 

  11. Lerner, A. M., Shippey, M. J., Crane, L. R.: Serologic responses to herpes simplex virus in rabbits: Complement requiring neutralizing, conventional neutralizing and passive hemagglutinating antibodies. J. inf. Dis.129, 623–636 (1974).

    Google Scholar 

  12. McClung, H., Seth, P., Rawls, W. E.: Quantitation of antibodies to herpes simplex virus types 1 and 2 by complement dependent antibody lysis of infected cells. Amer. J. Epidemiol.104, 181–191 (1976).

    Google Scholar 

  13. McClung, H., Seth, P., Rawls, W. E.: Relative concentrations in human sera of antibodies to cross reacting and specific antigens of herpes simplex virus types 1 and 2. Amer. J. Epidemiol.104, 192–201 (1976).

    Google Scholar 

  14. McMillan, B. C., Golubjatnikov, R., Hanson, R. P., Sinha, S. K.: A study of cytomegalovirus, Epstein-Barr virus andHerpes virus hominis (types 1 and 2) antibody in institutionalized and noninstitutionalized children. Health Lab. Sci.14, 261–268 (1977).

    Google Scholar 

  15. Nahmias, A. J., Dowdle, W. R., Kramer, J. L., Luce, C. F., Mansour, S. C.: Antibodies toHerpes virus hominis types 1 and 2 in the rabbit. J. Immunol.102, 956–962 (1969).

    Google Scholar 

  16. Prakash, S. S., Seth, P.: Evaluation of indirect hemagglutination and its inhibition in the differentiation between antibodies to Herpes simplex virus types 1 and 2 for seroepidemiologie studies: Use of a II/I index threshold of 85 and an assay of type specific antibodies. J. inf. Dis.139, 524–528 (1979).

    Google Scholar 

  17. Rawls, W. E., Iwamoto, K., Adam, E., Melnick, J. L.: Measurement of antibodies to herpes virus types 1 and 2 in human sera. J. Immunol.104, 599–606 (1970).

    Google Scholar 

  18. Roane, P. R., Jr., Roizman, B.: Studies on the determinant antigens of viable cells. II. Demonstration of altered antigenic reactivity of HEp-2 cells infected with Herpes simplex virus. Virology22, 1–9 (1964).

    Google Scholar 

  19. Roizman, B., Spring, S. B.: Alterations in immunologic specificity of cells infected with cytolytic viruses. In: Cross reacting antigens and neoantigens, 85–97. Baltimore, Md.: Williams & Wilkins Co. 1967.

    Google Scholar 

  20. Seth, P., Kesavalu, L.: Typing Herpes simplex virus antibodies by complement dependent antibody lysis of infected cells. Indian J. med. Res.71, 329–336 (1980).

    Google Scholar 

  21. Seth, P., Prakash, S. S., Choudhry, N., Ghosh, D., Bhargava, V. L., Mohapatra, L. N.: Followup studies on Herpes simplex type 1 and type 2 antibodies in patients with carcinoma of cervix. (Manuscript under preparation.)

  22. Seth, P., Prakash, S. S., Ghosh, D.: Antibodies to Herpes simplex types 1 and 2 in patients with squamous cell carcinoma of uterine cervix in India. Int. J. Cancer22, 708–714 (1978).

    Google Scholar 

  23. Seth, P., Prakash, S. S., Kesavalu, L.: Indirect hemagglutination test in typing Herpes simplex virus and antibodies. Indian J. med. Res.68, 887–895 (1978).

    Google Scholar 

  24. Seth, P., Rawls, W. E., Duff, R., Rapp, F., Adam, E., Melnick, J. L.: Antigenic differences between isolates of Herpes virus type 2. Intervirol.3, 1–14 (1974).

    Google Scholar 

  25. Smith, J. W., Adam, E., Melnick, J. L., Rawls, W. E.: Use of 51-Chromium release test to demonstrate patterns of antibody response in humans to Herpes virus types 1 and 2. J. Immunol.109, 554–564 (1972).

    Google Scholar 

  26. Thiry, L., Sprecher-Goldberger, S., Fassin, Y., Gould, I., Gompel, C., Perstian, J., de Halleux, F.: Variation of cytotoxic antibodies to cells with herpes simplex virus antigens with progressing cancerous lesions of the cervix. Amer. J. Epidemiol.100, 251–261 (1974).

    Google Scholar 

  27. Yang, J. P. S., Wentwroth, B. B.: Study of immune cytotoxicity withHerpes virus hominis infected cells. Proc. Soc. exp. Biol. Med.141, 759–764 (1972).

    Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India

    L. Kesavalu & P. Seth

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  1. L. Kesavalu
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  2. P. Seth
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Kesavalu, L., Seth, P. Comparison of indirect hemagglutination and51Chromium release tests for detection of herpes simplex virus types 1 and 2 antibodies in patients with recurrent herpes infections. Archives of Virology 66, 77–88 (1980). https://doi.org/10.1007/BF01314976

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  • DOI: https://doi.org/10.1007/BF01314976

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