Skip to main content
Log in

Adaptation of bluetongue virus in mosquito cells results in overexpression of NS3 proteins and release of virus particles

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Adaptation of bluetongue virus (BTV) to grow in mosquito cells (C6/36) resulted in overexpression of two non-structural proteins (NS3 and NS3a) in infected cells. These proteins also co-purified with BTV particles and were dissociated from the virions upon treatment with an anionic detergent. The expression was not host dependent, since back inoculation of the adapted virus into mammalian cell cultures also resulted in a significant overexpression of these proteins. The BTV-C6/36 produced smaller plaques in Vero cells compared with the parent strain. This is the first report which demonstrates a high level of NS3/NS3a expression in infected cells and subsequent release of infectious BTV particles into the supernatant.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. French TJ, Inumaru S, Roy P (1989) Expression of two related nonstructural proteins of bluetongue virus (BTV) type 10 in insect cells by a recombinant baculovirus: production of polyclonal ascitic fluid and characterization of the gene product in BTV-infected BHK cells. J Virol 63: 3270–3278

    Google Scholar 

  2. French TJ, Roy P (1990) Synthesis of bluetongue virus (BTV) core-like particles by a recombinant baculovirus expressing the two major structural core proteins of BTV. J Virol 64: 1530–1536

    Google Scholar 

  3. Gould AR (1988) Nucleotide sequence of the Australian bluetongue virus serotype 1 RNA segment 10. J Gen Virol 69: 945–949

    Google Scholar 

  4. Huismans H (1979) Protein synthesis in bluetongue virus infected cells. Virology 92: 385–396

    Google Scholar 

  5. Huismans H, Els H (1979) Characterization of the microtubules associated with the replication of three different orbiviruses. J Virol 92: 397–405

    Google Scholar 

  6. Hyatt AD, Zhao Y, Roy P (1993) Release of bluetongue virus-like particles from insect cells is mediated by BTV nonstructural protein NS3/NS3A. Virology 193: 592–603

    Google Scholar 

  7. Hyatt AD, Gould AR, Coupar B, Eaton BT (1991) Localization of the non-structural protein NS3 in bluetongue virus-infected cells. J Gen Virol 72: 2263–2267

    Google Scholar 

  8. Lee JW, Roy P (1986) Nucleotide sequence of cDNAs clone of RNA segment 10 of bluetongue virus (serotype 10). J Gen Virol 69: 2833–2837

    Google Scholar 

  9. Libikova H, Buckly SM (1971) Studies with Kemerovo virus in Singh'sAedes cell lines. Acta Virol 15: 393–403

    Google Scholar 

  10. Martyn JC, Gould AR, Eaton BT (1990) High level expression of the major core protein VP7 and the non-structural protein NS3 of bluetongue virus in yeast: use of expressed VP7 as a diagnostic, group-reactive antigen in a blocking ELISA. Virus Res 18: 165–178

    Google Scholar 

  11. Mertens PPC, Brown F, Sangar DV (1984) Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode. Virology 135: 207–217

    Google Scholar 

  12. Mertens PPC, Burroughs JN, Anderson J (1987) Purification and properties of virus particles, infectious subviral particles, and cores of bluetongue virus serotype 1 and 4. Virology 157: 375–386

    Google Scholar 

  13. Pudney M (1987) In: Yunker CE (ed) Arboviruses in arthropod cells in vitro, vol. 1. CRC Press, Boca Raton, pp 87–101

    Google Scholar 

  14. Rehacek J (1987) In: Yunker CE (ed) Arboviruses in arthropod cells in vitrol, vol 1. CRC Press, Boca Raton, pp 115–132

    Google Scholar 

  15. Urakawa T, Roy P (1988) Bluetongue virus tubules made in insect cells by recombinant baculovirus: Expression of NS1 gene of bluetongue virus serotype 10. J Virol 62: 3919–3927

    Google Scholar 

  16. Van Staden V, Huismans H (1991) A comparison of the genes which encode non-structural protein NS3 of different orbiviruses. J Gen Virol 72: 1073–1079

    Google Scholar 

  17. Van Dijk AA, Huismans H (1988) In vitro transcription and translation of bluetongue virus mRNA. J Gen Virol 69: 573–581

    Google Scholar 

  18. WU X, Chen SY, Iwata H, Compans RW, Roy P (1992) Multiple glycoproteins synthesized by the smallest RNA segment (S10) of bluetongue virus. J Virol 66: 7104–7112

    Google Scholar 

  19. Wechsler SJ, McHolland LE, Tabachnick WJ (1989) Cell lines fromCulicoide variipennis (Diptera: Ceratopogonidae) support replication of bluetongue virus. J Invertebr Pathol 54: 385–393

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Guirakhoo, F., Catalan, J.A. & Monath, T.P. Adaptation of bluetongue virus in mosquito cells results in overexpression of NS3 proteins and release of virus particles. Archives of Virology 140, 967–974 (1995). https://doi.org/10.1007/BF01314973

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01314973

Keywords

Navigation