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Affinity chromatography of viroid RNAs

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Summary

The application of a structure- and GC-specific affinity adsorbent for the purification of viroid RNAs is described. Cross-linked bisacrylamide gels to which the intercalating dye phenyl neutral red is covalently attached [2] constitute a powerful tool for the rapid, quantitative, and selective isolation of viroid as well as viroid complementary RNAs from crude, plant polysaccharides containing nucleic acid fractions.

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References

  1. Alwine, J. C., Kemp, D. J., Stark, G. R.: Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc. Natl. Acad. Sci. U.S.A.74, 5350–5354 (1977).

    Google Scholar 

  2. Bünemann, H., Müller, W.: Base specific fractionation of double stranded DNA: affinity chromatography on a novel type of adsorbent. Nucleic Acid Res.5, 1059–1074 (1978).

    Google Scholar 

  3. Diener, T. O.: Viroids and viroid diseases. New York: Wiley Interscience 1979.

    Google Scholar 

  4. Grill, L. K., Negruk, V. I., Semancik, J. S.: Properties of the complementary RNA sequences associated with infection by the citrus exocortis viroid. Virology107, 24–33 (1980).

    Google Scholar 

  5. Gross, H. J., Riesner, D.: Viroids: a class of subviral pathogens. Angew. Chem. Int. Ed. Engl.19, 231–243 (1980).

    Google Scholar 

  6. Riesner, D., Henco, K., Rokohl, U., Klotz, G., Kleinschmidt, A. K., Domdey, H., Jank, P., Gross, H. J., Sänger, H. L.: Structure and structure formation of viroids. J. mol. Biol.133, 85–115 (1979).

    Google Scholar 

  7. Rohde, W., Sänger, H. L.: Detection of RNA intermediates of viroid replication by Northern blot hybridization. Biosc. Rep.1, 327–336 (1981).

    Google Scholar 

  8. Rohde, W., Schnölzer, M., Rackwitz, H. R., Haas, B., Seliger, H., Sänger, H. L.: Specifically primed synthesisin vitro of full-length DNA complementary to potato spindle tuber viroid. Eur. J. Biochem.118, 151–157 (1981).

    Google Scholar 

  9. Rohde, W., Schnölzer, M., Sänger, H. L.: Sequence-specific priming of thein vitro synthesis of DNA complementary to citrus exocortis viroid. FEBS Lett.130, 208–212 (1981).

    Google Scholar 

  10. Sänger, H. L., Klotz, G., Riesner, D., Gross, H. J., Kleinschmidt, A. K.: Viroids are single-stranded covalently closed circular RNA molecules existing as highly basepaired rod-like structures. Proc. Natl. Acad. Sci. U.S.A.73, 3852–3856 (1976).

    Google Scholar 

  11. Sänger, H. L.: Structure and function of viroids. In:Prusiner, S. B., Hadlow, W. J. (eds.), Slow transmissible diseases of the nervous system, Vol. II, 291–341. London: Academic Press 1979.

    Google Scholar 

  12. Sänger, H. L., Ramm, K., Domdey, H., Gross, H. J., Henco, K., Riesner, D.: Conversion of circular viroid molecules to linear strands. FEBS Lett.99, 117–122 (1979).

    Google Scholar 

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Authors and Affiliations

  1. Arbeitsgruppe Pflanzenvirologie, Fachbereich Angewandte Biologie, Justus-Liebig-Universität, Giessen, Germany

    W. Rohde

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  1. W. Rohde
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Rohde, W. Affinity chromatography of viroid RNAs. Archives of Virology 71, 169–176 (1982). https://doi.org/10.1007/BF01314886

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  • DOI: https://doi.org/10.1007/BF01314886

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