Summary
Cowpox virus (CPV) growth and its S-ag (cell surface antigen) formation in HVJ (Sendai virus) carrier cells pre-treated with Actinomycin D or Puromycin were not affected as much as those in parent cells. These suggest the different cellular functions of carrier cells. The activity of carrier cell extracts causing a characteristic degradation of CPV reacted with themin vitro disappeared after the pre-incubation of extracts with hemoglobin or casein. Measurements of cellular protease activities including lysosomal enzymes demonstrated significant increases in the carrier cell extracts compared to those in parent cells. The CPV, thus reactedin vitro with the extracts or lysosomal fraction from carrier cells, acquired more rapidly and markedly the enhanced ability of S-ag formation parallel to virus infectivity alteration in the reaction.
These results indicate that the enhancement of CPV S-ag formation in the HVJ carrier cells may be due to their increased cellular enzymes, possibly proteolytic ones, capable of promoting the first step of CPV uncoating or degradation in the cells.
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References
Allison, A. C., Sandelin, K.: Activation of lysosomal enzymes in virus-infected cells and its possible relationship to cytopathic effect. J. exp. Med.117, 879–887 (1963).
Blackman, K. E., Bubel, H. C.: Poliovirus induced cellular injury. J. Virol.4, 203–208 (1969).
Defendi, V.: Cytopathology of virus infection. Fed. Proc. Amer. Soc. exp. Biol.21, 1113–1117 (1962).
Easterbrook, K. B.: Controlled degradation of vaccinia virionsin vitro: an electron microscopic study. J. ultrastruct. Res.14, 484–496 (1966).
Flanagan, J. F.: Hydrolytic enzymes in KB cells infected with poliovirus and herpes simplex virus. J. Bacteriol.91, 789–797 (1966).
Guskey, L. E., Smith, P. C., Wolff, D. A.: Patterns of cytopathology and lysosomal enzyme release in polio-infected HEP-2 cells treated either 2-(α-hydroxybenzyl)-benzinmidazole or guanidine HCL. J. gen. Virol.6, 151–161 (1970).
Hatano, M., Morita, O.: Multiplication and plaque assay of influenza viruses in a continuous cell line (G2) of human origin. Arch. ges. Virusforsch.20, 305–313 (1967).
Joklik, W. K.: The poxviruses, Ann. Rev. Microbiol.22, 359–390 (1968).
Joklik, W. K.: The preparation and characteristics of highly purified radioactively labeled poxvirus. Biochim. biophys. Acta61, 290–301 (1962).
Koschel, K., Aus, H. M., Ter Meulen, V.: Lysosomal enzyme activity in poliovirus-infected HeLa cells and vesicular stomatitis virus-infected L cells: biochemical and histochemical comparative analysis with computer-aided techniques. J. gen. Virol.25, 359–369 (1974).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with Folin phenol reagent. J. biol. Chem.193, 265–275 (1951).
Maeno, K., Yoshi, S., Nagata, I., Matsumoto, T.: Growth of Newcastle diseases virus in a HVJ carrier culture of HeLa cells. Virology29, 255–263 (1966).
Miyamoto, H., Kato, S.: Immune hemadsorption by cells infected with poxviruses. Biken's J.11, 343–353 (1968).
Miyamoto, H., Kato, S.: Cell surface antigens induced by poxviruses. I. Effect of antimetabolites on cell surface antigens. Biken's J.14, 311–324 (1971).
Sarov, I., Joklik, W. K.: Characterization of intermediates in the uncoating of vaccinia virus DNA. Virology50, 593–602 (1972).
Satoh, T., Yamamoto, N.: Repair mechanism in Sendai virus carrying HeLa cells after damage by 4-hydroxyaminoquinolie 1-oxide. Cancer Res.32, 440–443 (1972).
Tanaka, J., Hatano, M.: The effect of trypsin on the formation of virus-specific surface antigen in cowpox virus-infected cells. J. gen. Virol.21, 413–416 (1973).
Tanaka, J., Morita, M., Hatano, M.: Factors involved in the expression of poxvirus specific antigen in the Sendai virus carrier cells. J. gen. Virol.33, 87–99 (1976).
Ueda, Y., Ito, M., Tagaya, I.: A specific surface antigen induced by poxvirus. Virology38, 180–182 (1969).
Ueda, Y., Tagaya, I., Amano, H., Ito, M.: Studies on the early antigens induced by vaccinia virus. Virology49, 794–800 (1972).
Yamada, T., Hatano, M.: Lowered transplantability of cultured tumor cells by persistent infection with paramyxovirus (HVJ). GANN.63, 647–655 (1972).
Yamamoto, N., Morita, O., Satoh, T.: Effect of ultraviolet light and a potent carcinogen, 4-nitroquinoline 1-oxide, on mammalian cells and their Sendai virus carrier cultures. In:Beers, R. F., Jr., Heriatt, R. M., Tilgham, R. C. (eds.), Molecular and Cellular Repair Processes, 248–253. Baltimore: Johns Hopkins Univ. Press 1972.
Walter, K., Schutt, C.: In:Bergmeyer, H. U. (ed.), Methods of Enzymatic Analysis, 856–860. New York-London: Academic Press 1974.
Wolff, D. A., Bubel, H. C.: The disposition of lysosomal enzymes as related to specific viral cytopathic effects. Virology24, 502–505 (1964).
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Tanaka, J., Ogura, H. & Hatano, M. Cellular proteases increased in paramyxovirus (Sendai virus) carrier cells possibly responsible for enhanced formation of cowpox virus-specific cell surface antigen. Archives of Virology 53, 87–99 (1977). https://doi.org/10.1007/BF01314850
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DOI: https://doi.org/10.1007/BF01314850