Summary
Supercoiled infectious DNA was isolated from nuclear polyhedrosis virus infecting great wax moth (Galleria mellonella L.). Covalently closed DNA molecules constitute approximately 10–30 per cent in our preparations. These molecules dissappear during storage. Electron micrographs of supercoiled and open circular molecules are presented. Length of the open rings is about 50–52 µ. Infectivity of different DNA forms is discussed.
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Crawford, L. V.: The structure and function of viral nucleic acids. In:Crawford, L. V., Stoker, M. G. P. (eds.), The molecular biology of viruses, 163–181. Cambridge: Univ. Press 1968.
Graham, F. L., van der Eb, A. J.: A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology52, 456–467 (1973).
Hickson, F. T., Roch, T. F., Helinski, D. R.: Circular DNA forms of a bacteria sex factor. Proc. Nat. Acad. Sci. (Wash.)58, 1731–1738 (1967).
Hirt, B.: Selective extraction of Polyoma DNA from infected mouse cell cultures. J. mol. Biol.26, 365–369 (1967).
Kleinschmidt, A. K., Zahn, R. K.: Über DNA-Moleküle in Protein-Mischfilmen. Z. Naturforsch.14b, 770–779 (1959).
Kok, I. P., Chistiakova-Ryndich, A. V., Gudz-Gorban, A. P.: On the macromolecular structure of nuclear polyhedrosis virus of the silkworm. Molekuliarnaya biologia6, 323–331 (1972).
Nikonenko, V. U., Landau, S. M., Skuratovskaya, I. N., Kok, I. P.: Infectivity of DNA isolated from Galleria mellonella nuclear polyhedrosis virus. In: Proceedings of the conference on veterinary virology. 166–167, Moscow 1973.
Radloff, R., Bauer, W.:Vinograd, J.: A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc. Nat. Acad. Sci. (Wash.)57, 1514–1521 (1967).
Summers, M. D., Anderson, D. L.: Characterization of deoxyribonucleic acid isolated from the granulosis viruses of the cabbage looper, Trichoplusia ni, and the fall army-worm, Spodoptera frugiperda. Virology50, 459–471 (1972).
Summers, M. D., Anderson, D. L.: Characterization of nuclear polyhedrosis virus DNAs. J. Virol.12, 1336–1346 (1973).
Trkula, D., Kit, S., Hurimura, T., Nakajima, K.: Infectivity of molecular forms of simian virus 40 DNA. J. gen. Virol.10, 221–229 (1971).
Vinograd, J., Lebowitz, J., Radloff, R., Watson, R., Laipis, P.: The twisted circular form of polyoma viral DNA. Proc. Nat. Acad. Sci. (Wash.)53, 1104–1111 (1965).
Weil, R.: The denaturation and the renaturation of the DNA of Polyoma virus. Proc. Nat. Acad. Sci. (Wash.)49, 480–487 (1963).
Zaenen, I., van Larebeke, N., Teuchy, H., van Montagu, M.: Supercoiled circular DNA in crown-gall inducing Agrobacterium tume-faciens. J. mol. Biol.86, 109–127 (1974).
Zherebtsova, E. N., Strokovskaya, L. I., Gudz-Gorban, A. P.: Subviral infectivity in nuclear polyhedrosis of the great wax moth(Galleria mellonella L.). Acta virol.16, 427–431 (1972).
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Skuratovskaya, I.N., Strokovskaya, L.I., Zherebtsova, E.N. et al. Supercoiled DNA of nuclear polyhedrosis virus ofGalleria mellonella L. . Archives of Virology 53, 79–86 (1977). https://doi.org/10.1007/BF01314849
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DOI: https://doi.org/10.1007/BF01314849