Résumé
Ce travail étudie, au cours de la grippe expérimentale de la souris, l'évolution de la concentration en immunoglobulines (Ig) du sérum et des sécrétions bronchiques à l'aide de l'immunodiffusion radiale simple et l'évolution des titres d'anticorps spécifiques mesurés dans ces classes d'Ig par l'immunofluorescence. Les IgA bronchiques, non détectées chez l'animal normal, sont trois fois moins abondantes que les IgG chez l'animal infecté. Elles augmentent passagèrement. L'augmentation de la teneur des sécrétions bronchiques en IgG1 et IgG2, plus durable, est particulièrement forte pour les IgG1. La concentration maximale de ces trois classes d'Ig au 7ème jour peut impliquer une exsudation sérique. Les IgM, non dosables dans les sécrétions bronchiques normales, sont détectées chez l'animal infecté par l'immunofluorescence. Cette dernière montre qu'il y a des anticorps spécifiques dans chacune des quatre classes d'Ig. Les IgG2 et les IgM contiennent la plus forte concentration d'anticorps, les IgA en contiennent nettement moins. Dans chaque classe d'Ig, la comparaison du rapport concentration en Ig/titre d'anticorps dans le sérum et dans les sécrétions bronchiques montre qu'une grande partie de ces anticorps bronchiques est probablement synthétisée localement. Il n'a pas été possible de mettre en évidence une réaction anamnestique humorale locale.
Summary
Sera and bronchial washings from normal mice and from mice previously infected with influenza virus were analyzed for their concentration of four immunoglobulin (Ig) classes by the method of single radial immunodiffusion and for their content of specific antibody of these Ig classes by the immunofluorescent method. IgA were not detected in bronchial washings from normal mice. The IgA/IgG ratio was not higher than 0.33 in infected mice. The IgA level increased briefly in infected bronchial washings, but the levels of IgG2 and particularly of IgG1 exhibited a longer and higher increase. The maximal increase of these three Ig classes occurred by the seventh day and might have derived from transsudated serum. IgM was not identified in normal bronchial washings, but immunofluorescence detected IgM in infected washings, which also showed specific antibody in each of the four Ig classes. IgG2 and IgM contained antibody in greatest, IgA in lower concentration. Our results indicate that the most part of bronchial antibody was produced locally, since the ratio of Ig concentration to antibody titre of each Ig class was consistently higher for bronchial washings than for sera. An anamnestic secretory antibody response could not be demonstrated in bronchial washings.
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Bibliographie
Alford, R. H., Rossen, R. D., Butler, W. T., Kasel, J. A.: Neutralizing and hemagglutination-inhibiting activity of nasal secretions following experimental human infection with A 2 influenza virus. J. Immunol.98, 724–731 (1967).
Berendt, R. F.: Simian model for the evaluation of immunity to influenza. Infect. Immun.9, 101–105 (1974).
Brown, G. C., O'Leary, T. P.: Fluorescent antibodies to influenza virus in various immunoglobulin fractions of serum after natural infection or vaccination. J. Immunol.110, 889–896 (1973).
Fazekas de St. Groth, S., Donnelley, M.: Studies in experimental immunology of influenza. III. The antibody response. Aust. J. exp. Biol. Med. Sci.28, 45–60 (1950).
Haff, R. F., Pinto, C. A.: Comparison of neutralizing activity in nasal secretion and serum of ferrets in response to infection with influenza A viruses. Arch. ges. Virusforsch.40, 168–170 (1973).
Havez, R., Hayem, A., Laine, A., Lebas, J., Servais, V., Bonte, M.: Techniques de dosage des IgA (étude critique). Rev. Franç. Mal. Resp. (Paris)1, 877–992 (1973).
Lemercier, G., Schmitt, D., Quenin, P., Jayne, A., Fontanges, R.: La réponse immunitaire locale au cours de la grippe expérimentale de la souris Balb/c infectée par Myxovirus influenzae A/Hong Kong/1/68 (H3N2). Arch. ges. Virusforsch.45, 113–121 (1974).
Lowry, O. H., Rosenbrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951).
Mancini, G., Carbonara, A. O., Heremans, J. F.: Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochem.2, 235–246 (1965).
Osebold, J. W., Medin, N. I., Zee, Y. C.: Immunochemical analysis of serum related proteins in the respiratory tract secretions of normal mice. Infect. Immun.12, 1141–1146 (1975).
Potter, C. W., Shore, S. L., MacLaren, C.: Immunity to influenza in ferrets. III. Proteins in nasal secretions. J. inf. Dis.126, 387–393 (1972).
Schmitz, H., Haas, R.: Determination of different cytomegalovirus immunoglobins (IgG, IgA, IgM) by immunofluorescence. Arch. ges. Virusforsch.37, 131–140 (1972).
Shore, S. L., Potter, C. W., MacLaren, C.: Immunity to influenza in ferrets. IV. Antibody in nasal secretions. J. inf. Dis.126, 394–400 (1972).
Waldman, R. H., Jurgensen, P. F., Olson, G. N., Ganguly, R., Johnson, J. E.: Immune response of the human respiratory tract. I. Immunoglobulin levels and influenza virus vaccine antibody response. J. Immunol.111, 38–42 (1973).
Waldman, R. H., Mann, J. J., Kasel, J. A.: Influenza virus neutralizing antibody in human respiratory secretions. J. Immunol.100, 80–85 (1968).
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Lemercier, G., Neveu, D., Morel, J. et al. La Réponse Immunitaire Locale au Cours de la Grippe Expérimentale de la Souris: Immunoglobulines, Anticorps Spécifiques et Réponse Anamnestique des Sécrétions Bronchiques. Archives of Virology 53, 45–53 (1977). https://doi.org/10.1007/BF01314846
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DOI: https://doi.org/10.1007/BF01314846