Summary
Genomes of six virulent and three attenuated vaccine strains of pseudorabies (Aujeszky's disease) virus were compared by cleavage with five restriction endonucleases. Differences either in number or in size of DNA fragments could be detected among all nine strains.
References
Aujeszky, A.: Über eine neue Infektionskrankheit bei Haustieren. Zbl. Bakt. I. Abt. Orig.32, 353–373 (1902).
Bartha, A.: Experimental reduction of virulence of Aujeszky's disease virus. Magy. Allatorv. Lapja16, 42–45 (1961).
Bartha, A., Belak, S., Benyeda, J.: Trypsin- and heat-resistance of some strains of the herpesvirus group. Acta Vet. Acad. Sci. Hung.19, 97–99 (1969).
Engels, M., Steck, F., Wyler, R.: Comparison of the genomes of infectious bovine rhinotracheitis and infectious pustular vulvovaginitis virus strains by restriction endonuclease analysis. Arch. Virol.67, 169–174 (1981).
Hayward, G. S., Frenkel, H. G., Roizman, B.: Anatomy of herpes simplex virus DNA: Strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites. Proc. Natl. Acad. Sci. U.S.A.72, 1768–1772 (1975).
Lloyd, G., Baskerville, A.:In vitro markers to differentiate an avirulent from a virulent strain of Aujeszky's disease virus. Vet. Microbiol.3, 65–70 (1978).
Pirtle, E. C.: Variation in the modal chromosome number of two PK-15 porcine kidney cell lines. Am. J. Vet. Res.27, 747–749 (1966).
Platt, K. B., Mare, C. J., Hinz, P. N.: Differentiation of vaccine strains and field isolates of pseudorabies (Aujeszky's disease) virus: Thermal sensitivity and rabbit virulence markers. Arch. Virol.60, 13–23 (1979).
Platt, K. B., Mare, C. J., Hinz, P. N.: Differentiation of vaccine strains and field isolates of pseudorabies (Aujeszky's disease) virus: Trypsin sensitivity and mouse virulence markers. Arch. Virol.63, 107–114 (1980).
Roizman, B.: Genome variation and evolution among herpes viruses. Annals. New York Acad. Sci.354, 472–483 (1980).
Saunders, J. R., Gustafson, D. P., Olander, H. J., Jones, R. K.: An unusual outbreak of Aujeszky's disease in swine. Proc. 67th Annu. Meet. U.S. Livestock Sanit. Assoc. 331–346, Albuquerque, New Mexico 1963.
Scherba, G., Gustafson, D. P., Kanitz, C. L., Sun, I. L.: Delayed hypersensitivity reaction to pseudorabies virus as a field diagnostic test in swine. J. Am. Vet. Med. Assoc.173, 1490–1493 (1978).
Shope, R. E.: An experimental study of mad itch with special reference to its relationship to pseudorabies. J. Exp. Med.45, 233–248 (1931).
Skoda, R., Brauner, I., Sádecký, E., Mayer, V.: Immunization against Aujeszky's disease with live vaccine. I. Attenuation of virus and some properties of attenuated strains. Acta Virol.8, 1–9 (1964).
Studdert, M. J., Simpson, T., Roizman, B.: Differentiation of respiratory and abortigenic isolates of equine herpesvirus 1 by restriction endonucleases. Science214, 562–564 (1981).
Swaney, L. M.: Susceptibility of a new fetal pig kidney cell line (MVPK-1) to foot-and-mouth disease virus. Am. J. Vet. Res.37, 1319–1322 (1976).
Walboomers, J. M. M., Schegget, J. T.: A new method for the isolation of herpes simplex virus type 2 DNA. Virology74, 256–258 (1976).
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Paul, P.S., Mengeling, W.L. & Pirtle, E.C. Differentiation of pseudorabies (Aujeszky's disease) virus strains by restriction endonuclease analysis. Archives of Virology 73, 193–198 (1982). https://doi.org/10.1007/BF01314727
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DOI: https://doi.org/10.1007/BF01314727